Research

Funding: Drittmittelprojekt, gefördert durch Fritz-Ahrberg-Stiftung., 67.500 EUR

Project Details:
Sous vide cooking is the heating of raw ingredients under controlled temperature-time combinations in heat-stable vacuum bags. As with all LTLT cooking methods, the choice of a suitable temperature-time combination is the most important parameter for microbiologically safe food in sous vide-cooking. The aim of this study is to define microbiologically safe temperature-time combinations for the inactivation of pathogens in sous vide-cooked minced beef and pork. In addition, a characterization of technological and sensory aspects of the influence of the sous vide cooking method on hamburger patties is carried out.

Funding: Schaumann Stiftung, 5.000 EUR

Project Details:
Die Leber des Milchkuh ist aufgrund der hohen Milchleistung der Tiere ein wichtiges Stoffwechselorgan. Um detallierte Mechanismen erforschen zu können, soll in diesem Projekt bovine embryonale Zellen zu Hepatozyten differenziert werden. Der große Vorteil stammzellbasierter Hepatozytenmodelle ist, dass man nicht abhängig von gesunden Spenderorganismen ist, aus denen funktionsfähige primäre Hepatozyten isoliert werden können. Im
Rahmen verschiedener Projekte wurden bereits unterschiedliche Protokolle beschrieben, um nach einer in-vitro Differenzierung sowohl morphologisch als auch funktionell möglichst Hepatozyten-ähnliche Zellen ("Hepatocyte like-cells", HLC) zu generieren. Hierbei wurden Stammzellen von z.B. Mäusen nach der Isolation mit
verschiedenen Wachstumsfaktoren, Zytokinen und anderen Faktoren kultiviert. Unter anderem kommt bei der
Differenzierung von Stammzellen zu Hepatozyten-ähnlichen Zellen der Fibroblast Growth Factor 2 (FGF-2),
Aktivin A, Hepatocyte Growth Factor (HGF) und Dexamethason zum Einsatz. Als Qualitätsmerkmale für die
stammzellbasierten Hepatozyten-ähnlichen Zellen werden die zelluläre Morphologie, sowie die Gen- und
Proteinexpression als funktionelle Komponente herangezogen. So sind diese Zellen in der Lage z.B. Albumin zu
produzieren. Ein weiterer großer Vorteil
dieser stammzellbasierten Hepatozyten ist die deutlich längere Lebensdauer im Vergleich zu den primären
Hepatozyten, welche in Kultur frühzeitig phänotypische Altersanzeichen zeigen und generell nur eine kurze
Überlebensdauer haben.

Funding: Gesellschaft zur Förderung kynologischer Forschung e.V., 11.520 EUR

Project Details:
Hypothyroidism is on of the the most commonly encountered endocrinopathy in dogs. However, low thyroxine concentrations are also found as adaptive mechanismen with other diseases (non-thyroidal illness syndrome, NTIS) and it is correlated with severity of diseases and thus, mortality. A discrimination between hypothyroidism and NTIS is not just viable for proper treatment but eminent for patient’s prognosis. Systemic inflammatory response syndrome (SIRS) and pro-inflammatory cytokines have been linked to the pathogenesis of the NTIS in both human and animal studies. The aim of this study was to characterize two common canine endocrinopathies (hypoadrenocorticism, hyperadrenocorticism) and their effects on the thyroid gland in the context of a possible NTIS. A NTIS is defined based on an advanced thyroid panel (TT3, fT3, T3RU, TNF-a, IL-1, and IL-2) in canine serum.
Methods In this retrospective cross-sectional study data and left-over of clinical diagnostic serum samples from dogs stratified as healthy, Cushing’s, or Addison diseased were assessed by a single German veterinary laboratory to avoid inter-assay and intra-assay variability via validated chemiluminescence assay and enzyme linked immunosorbent assay (ELISA [TgAA, IL-1, IL-2, TNF-a]) in accordance to the American Society of Veterinary Clinical Pathologists’ (ASVCP) guidelines for Quality Assurance in Laboratories.

Funding: MWK, 104.704 EUR

Project Details:
COFONI LZF-TP-Expansion to include the research area
"Interdisciplinary research into the long-term consequences of SARS-CoV-2 pandemic

Results:

https://www.umg.eu/forschung/corona/cofoni/

Funding: MWK via UMG (COFONI), 86.260 EUR

Project Details:
Type I interferon signaling and mitochondrial dysfunction in the heart acute and long-Covid disease.

Results:

https://www.umg.eu/forschung/corona/cofoni/

Funding: Industry (Animal breeding), 1.056.339 EUR

Funding: MWK, 104.704 EUR

Project Details:
COFONI-LPC TechnologiePlattform 2-
Animal models & test systems

Results:

https://www.umg.eu/forschung/corona/cofoni/

Funding: Drittmittelprojekt, gefördert durch die Fritz-Ahrberg-Stiftung., 17.500 EUR

Project Details:
Comparative tests are carried out with different sample materials. Fresh pork, chicken and beef are used, as well as meat products from the categories of raw and cooked sausage and ham (raw and cooked ham).
Three selected sample thicknesses are examined in each case, depending on commercially available cuts and products on offer, in order to be able to assess the influence of the material height on the subsequent analyses. The actual tests then include the measurement of color and texture.
With regard to the color measurement (instrumentally by means of chromameter), an interaction between sample material, sample thickness and background color is examined. The aim is to sensitize subsequent projects to possible influences and misinterpretations in connection with the methodological implementation.
When assessing the texture, various attachment tools are available for instrumental measurement using TA.XTplus, which are to be selected and compared depending on the respective material. The aim is to evaluate the necessity of the different preparation-intensive measurements and to identify comparable tools as well as to compare the measurement results of the different tools.

Project Details:
The project focuses on the isolation of phages from the feces of bearded dragons. After isolation, phages are examined and classified according to their efficacy and characteristics. Subsequently the influence of phage application on the microbiom and shedding of Salmonella is examined. These examinations are of relevance because of the zoonotic potential of Salmonella shedding in reptiles.

Cooperation Partners:

Robert-Koch-Institut Wernigerode

Project Details:
A blood test provides information on the physiological or pathological status of an animal. In veterinary medicine, another focus is on monitoring the nutritional status of a patient. The patient's results, obtained through sampling and appropriate analysis procedures, are categorised with previously established RI of a healthy reference population. The laboratory results provide important information for the diagnosis and prognosis of a sick animal. In addition to correct analysis, the reliability of laboratory results requires quality-assured pre-analysis and appropriate and evidence-based RI. Even accurate measurement results can lead to incorrect diagnosis and therapy if the sample has been altered at the time of analysis to such an extent that it no longer corresponds to the basic population of the previously used reference population. Many factors influence the quality of samples in the pre-analytical phase and the basis for assessment in the post-analytical phase. The aim of this project is therefore, for the first time, to focus significantly more on pre-analytical factors in the production of RI. In order to avoid diagnostic errors, pre-analytical workflows should be standardised as far as possible. According to a thorough literature search, the RIs of the following parameters in particular differ significantly from each other and will therefore be reproduced in this paper using a reference population. In particular, this will be done with very clearly defined pre-analytical conditions. From clinical chemistry, aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transferase (GGT), glutamate dehydrogenase (GLDH), creatine kinase (CK), total protein (TTP), albumin, total bilirubin, urea, beta-hydroxybutyrate (ß-HBS), free fatty acids (FFA), cholesterol and various minerals are considered.
The aim is to further develop and establish recommendations that can be implemented in daily practice and laboratory work in veterinary medicine, are based on current and quality-assured RI for dairy cows from Germany and lead to meaningful laboratory results.