Research

Funding: Drittmittelprojekt, gefördert durch die Deutsche Forschungsgemeinschaft (DFG)., 269.600 EUR

Project Details:
Bacillus cereus is a common soil bacterium responsible for two types of foodborne gastrointestinal diseases. The emetic variant leads to food poisoning and manifests in nausea and vomiting, while infections with enteropathogenic strains cause diarrhoea and abdominal pain. These symptoms are caused by various toxins, including the cyclic dodecadepsipeptide cereulide and the protein-based enterotoxins haemolysin BL (Hbl), non-haemolytic enterotoxin (Nhe) and cytotoxin K (CytK).
The principle of action of the pore-forming three-component toxin Hbl was investigated in detail in a previous project. The complex formation of the three protein components in solution, the binding order to the target cells, the optimum concentration ratio for fastest pore formation and maximum cytotoxicity, as well as the effect of a fourth protein component were determined. While the preliminary work focused on the toxin itself, this project investigates the effects of pore formation on the host.
This project provides answers to the cellular response of the primary target of B. cereus enterotoxins, namely human cells of the gastrointestinal tract. Firstly, relevant toxin concentrations and exposure times for the survival of intestinal cells or the onset of apoptosis will be determined. In addition, the onset of possible repair mechanisms within the target cells is investigated. These initial data already provide a model for the onset of diarrhoea caused by enteropathogenic B. cereus. Following these studies, the interaction of B. cereus and its enterotoxins with more complex structures, namely human intestinal organoids, is investigated. The use of organoid culture systems enables more elaborate studies on host-microorganism interactions. Various aspects are investigated, including gene expression, ion transport and the activation of signalling pathways within the target cells and structures.
Important new insights into the physiological processes that lead to enterotoxin-induced diarrhoea are expected.

Funding: cofinanziert durch die Europäische Kommission, 107.187 EUR

Project Details:
This project aims to address a critical gap in neurotoxicity assessment by developing a novel in vitro methodology specifically targeting myelin toxicity—a decisive factor influencing peripheral sensory and motor functions. Building upon established modes-of-action (MoA) in adult neurotoxicity, the study utilizes human induced pluripotent stem cells to differentiate into mature motor and sensory neurons, along with Schwann cells. Distinguishing itself from existing in vitro methods, the project focuses on myelin toxicity, an unexplored MoA not yet incorporated into existing approaches, thereby influencing neurotoxicity assessment.
Acknowledging the regulatory imperative for swifter and more human-relevant neurotoxicity evaluations, the project aims to deliver an effective myelin toxicity assessment method. The methodology involves co-cultivating sensory or motor neurons with Schwann cells in both 3D spheres and 2D configurations. The plan encompasses characterizing cell types and scrutinizing myelin formation through immunocytochemical stainings and RT-qPCR after 4-8 weeks in culture. A pivotal aspect of the study is the exploration of optimal conditions for high-throughput testing.
Milestones include achieving the expression of neuron and Schwann cell markers, determining the optimal 2D or 3D setup for automated high-throughput myelin quantification, and scientifically validating the test method. The ultimate objective is to furnish a robust tool for assessing myelin toxicity, utilizing a compound training set.
In conclusion, this project pioneers an approach to address the gap in myelin toxicity testing within in vitro neurotoxicity assessment. By leveraging human induced pluripotent stem cells and advanced co-culture techniques, the study aims to make a substantial contribution to the development of a more comprehensive and effective neurotoxicity evaluation testing battery for regulatory use.

Results:

https://www.eu-parc.eu/

Funding: Gefördert durch die Bundesanstalt für Landwirtschaft und Ernährung Bonn/BMEL, 369.000 EUR

Project Details:
Der innovative Ansatz dieses Projektes zielt darauf ab, aus der Produktionskette isolierte Phagen als nachhaltige prophylaktische Maßnahme in der Geflügelproduktion zu etablieren.
Es sollen im Rahmen eines Zweistufenkonzepts maßgeschneiderte Phagenprodukte entwickelt werden.

Cooperation Partners:

Tierärztliche Gemeinschaftspraxis WEK, Miavit GmbH

Funding: Bundesministerium für Ernährung und Landwirtschaft (BMEL), 365.439 EUR

Project Details:
Ziel des Projektes "CalPhy" ist es, das Auftreten von behandlungsbedürftigen Erkrankungen in der Kälberhaltung zu reduzieren und damit erhöhten Verlusten entgegen zu wirken. Durch den Einsatz von hochwertigen Ergänzungen soll insgesamt die Versorgung der Kälber verbessert und damit die Tiergesundheit, das Tierwohl und die Leistung gefördert werden. Gezielt soll für die besonders empfindlichen Tiere innerhalb der Tiergruppen ein teil-individualisiertes Fütterungskonzept für die Praxis entwickelt werden. Leichte und in Teilen auch in gut geführten Beständen durch unvermeidbare Situationen (mechanische Geburtsprobleme etc.) geschwächte Neugeborene, sind besonders betroffen von einer ungenügenden Kolostrumversorgung. Diese Kälber benötigen zusätzlichen Schutz, damit die Gefahr eines überdurchschnittlichen Abfalls des kolostralen Immunschutzes nicht die Infektionsanfälligkeit erhöht. Um das Risiko von Erkrankungen auf das unvermeidbare Minimum zu reduzieren, sind auf Basis einer sensiblen Erfassung von ersten Krankheitsanzeichen bzw. prädisponierenden Situationen weitere Maßnahmen sinnvoll. Futtermittelzusatzstoffe können zur Stärkung von Kälbern und damit Förderung ihrer Gesundheit eingesetzt werden. Insbesondere phytogene Zusatzstoffe haben in gut geführten Betrieben das Potential, die Notwendigkeit für antibiotische Behandlungen weiter zu reduzieren. Durch die Reduzierung der Häufigkeit von Erkrankungen in der Kälberaufzucht sowie damit verbunden eine Steigerung der Tiergesundheit und des Tierwohls, kann das Projekt einen substantiellen Beitrag zur Förderung der nachhaltigen Leistungsfähigkeit der Agrarwirtschaft bei bestmöglicher Förderung des Tierschutzes in der Landwirtschaft leisten.

Cooperation Partners:

Phytobiotics Futterzusatzstoffe GmbH

Funding: Nds. Ministerium für Wissenschaft und Kultur, 218.996 EUR

Project Details:
Ziel des Projektes "Future-VET B2" ist, neben der praxisnahen Ausbildung der Studierenden der Tiermedizin, Dienstleistung und Forschung der Transfer der Forschungsergebnisse in die Fachwelt und Öffentlichkeit. Die Maßnahme "Future-VET 2B" hat dabei das übergeordnete Ziel die Lebensmittelproduktion im Hinblick auf die Umweltauswirkungen und die Ernährungssicherheit für die wachsende Weltbevölkerung zu optimieren. Insekten können hierbei sowohl im Sinne der Kreislaufwirtschaft zusätzliche, wertvolle Futtermittel und Lebensmittel darstellen. Zudem bieten sie die Möglichkeit bei Vorliegen bestimmter funktioneller Eigenschaften als Futtermittel sowohl die Gesundheit von Tieren im Sinne der Verwendung von Futtermitteln für besondere Ernährungszwecke (VO (EU)2020/354) zu fördern, als auch nach entsprechender Modifikation eine Prüfung als Futtermittelzusatzstoff mit einer funktionellen Wirkung im Sinne der VO (EG) Nr. 1831/2003 nach Vorgabe der VO (EG) Nr. 429/2008 zu durchlaufen. Im Rahmen dieses Teilprojektes soll die bestehende Insektenzucht an der TiHo erweitert werden, um alternative Proteinquellen für Tier und Mensch zu erschließen, die
(1)Zusätzlich eine Sonderfunktion im Hinblick auf bestimmte gesundheitsbezogene Ernährungszwecke für das Tier (ParNuts in Analogie zu VO (EU) 354/2020) haben,
(2)Zusatzstofffunktion gemäß VO (EG) Nr. 1831/2003 erfüllen sollen und natürlichen Ursprungs sind und
(3)Am Ende des Weges sollen die bevorzugten Insektenarten als Novel Food (VO (EU) 2015/2283) auch Funktionen erfüllen können, die der nationalen Ernährungsstrategie der Bundesregierung und der Nationalen Reduktions- und Innovationsstrategie für Zucker, Fette und Salz in Fertigprodukten entsprechen.
Hierfür werden stufenweise, systematisch entsprechende Vorarbeiten durchgeführt, die die Möglichkeit einer Nutzung bzw. Bausteine einer Marktzulassung als alternative Proteinquelle für Tiere, Futtermittel mit bestimmten gesundheitsbezogenen Ernährungszwecken (ParNuts), Futtermittelzusatzstoff oder Novel Food ermöglichen bzw. werden sollen.

Funding: MWK, 540.878 EUR

Project Details:
Comprehensive characterization of the hormone profiles of the HPG axis in male and female Long COVID patients, hormone panels, hormone measurements and statistical evaluations.
Creation of expression profiles of sex hormones and
sex hormone receptors in the plasma, lungs, reproductive organs and brains of male and female hamsters.

Results:

https://www.umg.eu/forschung/corona/cofoni/

Funding: Bundesministerium für Ernährung und Landwirtschaft (BMEL), 164.593 EUR

Project Details:
The aim of "InUtero" is to develop a lifelike simulator for midwifery in small ruminants and to implement this model in teaching and training. The Clinical Skills Lab, together with the Clinic for Small Ruminants, is responsible for the development and validation of the simulator as well as the preparation of detailed instructions. The animal husbandry school at the Triesdorf Agricultural Training Institute and the Echem Agricultural Training Center will use and evaluate this simulator as part of the obstetric teaching of their trainees as well as in the context of seminars and workshops in adult education. The close cooperation between the project partners enables the simulator to be constantly adapted and modified so that at the end of the project phase there is a lifelike simulator for midwifery in small ruminants that is optimized to meet the demands and needs of both teachers and learners.

Cooperation Partners:

Landwirtschaftliches Bildungszentrum Echem

Landwirtschaftliche Lehranstalt Triesdorf

Funding: Industry (Stable equipment/Animal husbandry supplies), 196.665 EUR

Project Details:
Sensor-based devices are increasingly used to objectify lameness and other gait abnormalities in horses. Recording the ground reaction forces of the hooves is the gold standard but measurements require well equipped facilities and significant effort. With the help of pressure boots, which can be easily attached to the horse's hooves, their pressure on the ground and position during locomotion can be determined. Data will be compared to established kinetic (pressure measuring plate) and kinematic methods (Equinosis Lameness LocatorTM).

Results:

Keller, J., Jung, K., Geburek, F. Equine Lameness Detection and Monitoring with an Instrumented Hoof Boot.

In: Proceedings of the 5th Scientific Meeting of the European College of Veterinary Sports Medicine and Rehabilitation, ECVSMR; Cordoba, Spain, October 16-18, 2024; in print

 

Geburek, F., Jung, K., Keller, J. Bewegungsanalyse mit instrumentierten Hufschuhen - was ist möglich?

In: Tagungsband des DVG-Vet-Congress 2024 - 7. Internationaler Kongress zur Pferdemedizin / Tagung der DVG-Fachgruppe Pferdekrankheiten, 1.- 2. November 2024, Berlin, Verlag der DVG Service GmbH, Gießen, ISBN 978-3-86345-736-5, S. 44-46

 

Geburek, F. Objective gait analysis with instrumented hoof boots: What is possible?

In: British Equine Veterinary Association (BEVA) Congress 2025, September 10- 13, 2025, Birmingham, United Kingdom, p. 161

 

Keller, J. Validierung und klinische Anwendung eines mit Druck- und Lagesensoren ausgestatteten Hufschuhs bei Pferden. Dissertation, Tierärztl. Hochsch. Hannover, 2025; Cuvillier Verlag, Göttingen.

 

Geburek, F., Kopf, A.-K., Jung, K., Hassenstein, M., Keller, J. In-vivo validation of an instrumented hoof boot for gait analysis equipped with pressure sensors and an inertial measurement unit.

In: Proceedings of the 71st AAEP Annual Convention, December 6-10, 2025; American Association of Equine Practitioners, Denver, CO, USA, p. 90-91

Project Details:
The objective of this prospective randomised clinical trial is to evaluate the occurrence of post-operative ileus as well as the short- and long-term outcome with the use of two different suture patterns for jejunojejunal anastomosis. Furthermore, histomorphology and several markers for inflammation, cell death, the enteric nervous system and stem cells will be evaluated in intestinal samples taken from the resection margins. In cases necessitating anastomotic revision or euthanasia, this (immuno-)histological examination will also be performed on the anastomosis itself. Determining the expression and distribution of these markers will enable the characterisation of this tissue prior to and following anastomosis and possibly identify causes for post-operative ileus or anastomotic failure.

Funding: MEKUN, 132.092 EUR

Project Details:
Grey seals are increasingly found on both the North Sea and Baltic Sea coasts of Schleswig-Holstein, indicating that the number of animals using these waters is continuing to rise.
The increasing populations also increase the urgency of examining these marine mammals for their health status, as it is suspected that they can play a role in the spread of infectious diseases of harbor seals such as distemper, particularly due to their extended migratory behavior. In addition, individual animals with severe disease processes are repeatedly recorded in the Baltic Sea. In both waters, it is particularly important to examine the animals for their health status as carriers of zoonotic diseases, especially as people have been infected with these pathogens through contact with carcasses in the past. Furthermore, potential anthropogenic influences on disease incidence must be investigated in order to protect the seal populations.
With increasing populations, attacks by gray seals on other grey seals, harbor seals and harbor porpoises are also on the rise. This can occur through predation, but also through sexual intercourse with harbor seals as well as juvenile grey seals of both sexes. Negative population effects are possible as well as effects on the health of the marine mammal-eating grey seals, as they hunt at a higher trophic level than their conspecifics, comparable to polar bears or orcas. Initial cases of influenza in both harbor seals and grey seals have shown that seals are susceptible to this virus and need to be studied closely both as a reservoir and as a "spillover" of this potential zoonosis. In addition, anthropogenic activities in the habitat of grey seals continue to increase, which can have various influences not only on the health status but also on the habitat use of the animals.
The aim of this research project is to investigate the health status and causes of death of grey seals that died in the waters of Schleswig-Holstein. The aim is to examine whether the parasitic, viral and microbial load of grey seals, in particular zoonotic pathogens, is changing. For this purpose, the grey seals, which will be recovered in the years 2023 to 2026 and brought to the ITAW for examination, will be completely dissected. Histological, parasitological, virological and microbiological examinations will be carried out. In addition, a comparison will be made with the results from previous years in order to assess the development of the health status.
The development of grey seal populations in the North Sea and Baltic Sea will also be researched and evaluated. As grey seals are highly mobile marine mammals, data will be exchanged with neighboring countries. The Trilateral Wadden Sea Secretariat (CWSS) and the Trilateral Marine Mammal Expert Group (EGMama), as well as HELCOM's Marine Mammal Health Group for the Baltic Sea region and OSPAR's Marine Mammal Working Group in the North Sea, play a special role here. The results of the investigations are urgently needed for the work on the Marine Protection Framework Directive and the aforementioned agreements.