Research

Funding: Niedersächsisches Ministerium für Wissenschaft und Kultur, 178.000 EUR

Project Details:
Measure 1 consists of work package A, which is part of e-learning service. The use of the blended learning format in veterinary teaching is to be promoted. A further focus will be placed on strengthening the (e-)didactic skills of lecturers and the digital skills of students, for which training courses will be created for these target groups.

Measure 1 also includes work package B, which is located in the Clinical Skills Lab. The establishment of a surgical logbook is intended to strengthen students' surgical skills. To support the implementation of the surgical logbook, a training concept for teaching surgical skills is being developed.

Funding: Bundesministerium für Bildung und Forschung, 625.000 EUR

Project Details:
As long as animal experiments cannot be completely replaced, the question will arise on which animals research should take place. In this project, an interdisciplinary working group wants to investigate the ELSA-relevant question, according to which criteria the selection of laboratory animal models takes place or should take place. The aim of the enterprise is to determine the manifold reasons for the choice of certain laboratory animals; the process is interdisciplinary in the full sense of the word and put the different rationalities into a well-founded correlation. The question condenses several open and important issues concerning the treatment of animals. The answer is to be examined in each of the disciplines involved, between them and finally in their association, whether it is possible to establish a coherent catalog of criteria that also creates clarity for the practice of animal experimentation, both for everyday research and at the interface to legislation and enforcement. This is important against the background of rapidly changing social value judgements about animals, which also lead to permanent tensions here. In this project we want to investigate the ELSA-relevant question of the criteria for the selection of laboratory animal models. We follow an interdisciplinary approach, which is represented by the following cooperation partners: Its members are: Prof. Dr. Peter Kunzmann (ethics), Prof. Dr. Bernhard Hiebl (laboratory animal science), Prof. Dr. Maren von Köckritz-Blickwede (infection research) (all TiHo- Hannover) and the legal scientist Prof. Dr. Dr. Tade Spranger (University of Bonn).

Cooperation Partners:

Tade Spranger, Rechts- und Staatswissenschaftliche Fakultät, Bonn

Funding: DFG, 498.400 EUR

Project Details:
The equine recurrent uveitis (ERU) is the main reason for blindness in horses, and 10% of the european horses are affected. Significantly, ERU is an important available model for human autoimmune uveitis as the clinical and immune-pathological characteristics are similar. As a cause for ERU, various hypotheses are described in the literature. On the one hand, infections with Leptospira interrogans are discussed as well as other bacterial, parasitic or viral infections. Independent of the initiating cause, there are numerous indications that ERU is an immune-mediated disease. In the body after the control of the primary infection, an overreaction of the immune system occurs in response to activated immune cells. In addition to lymphocytes, these immune cells can also be neutrophil granulocytes. Among various antimicrobial functions, neutrophils have been shown to release neutrophil extracellular traps (NETs). NETs are extracellular DNA fibers with associated proteases and antimicrobial peptides which are released by activated cells. NETs mediate entrap of invading pathogens, but when massively released may contribute to detrimental autoimmune reactions of the host.
The research project presented here is intended to provide essential insights for the involvement of NETs in the pathogenesis of ERU. In the first phase of our study, our findings indicate an involvement of NETs in the pathogenesis of ERU with detrimental effects. In short, NETs correlate to disease severity, seem to damage the blood retinal cells, and induce autoantibodies against NET proteins. We hypothesize, that those autoantibodies might shield the NETs from degradation by DNases, resulting in their persistence and potentially in new disease episodes. Thus, in the current project we aim to characterize if and how the phase of disease (non-active quiescent phase versus acute inflammatory phase) influences cellular involvement and extracellular DNA release. Based on our previous data, we further hypothesize that different cell types besides neutrophils play a role in extracellular DNA release during the progression of ERU, which enables crosstalk between various immune cells. Therefore, we will study the release of ETs by other immune cells besides neutrophils and its interplay with adaptive immune cells. Finally, since the use of gentamicin in the treatment of ERU patients is well established, the aim is to determine the influence of gentamicin in varying concentrations and along with distinct stimuli on equine immune cells, focusing on ET release.
 

Funding: Niedersächsisches Ministerium für Wissenschaft und Kultur (MWK), 324.000 EUR

Project Details:
The aim of this subproject is the further development and optimization of a 3D lung epithelial cell culture model
for the investigation of viral and bacterial infections and the effect of immunomodulatory
immunomodulatory substances on the course of infection and cell biochemical processes. An increased
complexity of the model system using physiological or pathophysiological oxygen
conditions and microfluidic systems enables the in vitro system to be approximated to the in vivo situation and
to the in vivo situation and will help to reduce the number of test animals in the long term. The
complexity of the infection model is reduced by physiologically relevant oxygen conditions (defined
tissue-specific hypoxic conditions) and by 3D co-cultivation of human or animal pulmonary
or animal pulmonary epithelial cells and neutrophil granulocytes. In addition
the use of primary cells and induced pluripotent stem cells should provide long-term optimization.

Funding: Bundesanstalt für Landwirtschaft und Ernährung (BLE), 134.128 EUR

Project Details:
The overall goal of the project is that farms that have high antibiotic use (ABU) can learn from farms that have comparably low ABU and the identification and optimization of parameters to improve animal health. The specific objectives of the project are: to improve biosecurity and overall hygiene of broiler chicken farms, to improve animal health of broiler chickens, to minimize the use of antibiotics, to transfer knowledge between broiler chicken farms with low and high ABU, and to optimize risk areas based on knowledge transfer.
There should be an intensive exchange of experiences between farmers who have different levels of ABU within the project. Thus, farms that have a high need for antibiotic use at the beginning of the project will learn which parameters they can and should optimize to improve animal health and minimize antibiotic use. Farm management factors that have a potential impact on animal health and consequent necessary ABU will be evaluated and collected during the practical phases of the project. These factors are divided into respective work packages (WP), including biosecurity of the farm and hygiene management (worked on by FLI & UROS), cleaning and disinfection (UROS), animal health and stable or herd management (TiHo and LMU), and knowledge transfer (UROS).
The project thus involves farms that have already been able to minimize their antibiotic use through innovative measures, optimized management and improved husbandry conditions. In addition, farms that are interested in improving animal health and minimizing antibiotic use and want to learn from other farms are invited to participate.

Cooperation Partners:

Uni ROS; Prof. Helen Louton

LMU München; PD Dr. Elke Rauch

FLI Insel Riems; PD Dr. Nicolai Denzin

Project Details:
At Hofgut Neumühle, data on the health and performance of dairy cows is continuously collected and stored in various electronic data management systems. Scientific questions repeatedly arise that should be clarified with the help of an analysis of the available data (so-called secondary data analysis). Before this data analysis is possible, an overview of the available data sources, the primary collection objectives, the data contained and the metadata describing the existing data should be drawn up.
To this end, the origin of the data is first traced on site and the (original/primary) purpose for which it was collected is understood and documented. Extracts from the data sources are created to serve the creation of the above-mentioned over-view. These excerpts will be further analyzed using application examples. As an important aspect of the work, the implications for subsequent secondary data analyses will be presented and discussed (Translated with DeepL.com (free version)).

Cooperation Partners:

Hofgut Neumühle (Theresa Scheu)

Funding: Industry (Stable equipment/Animal husbandry supplies), 196.665 EUR

Project Details:
Sensor-based devices are increasingly used to objectify lameness and other gait abnormalities in horses. Recording the ground reaction forces of the hooves is the gold standard but measurements require well equipped facilities and significant effort. With the help of pressure boots, which can be easily attached to the horse's hooves, their pressure on the ground and position during locomotion can be determined. Data will be compared to established kinetic (pressure measuring plate) and kinematic methods (Equinosis Lameness LocatorTM).

Results:

Keller, J., Jung, K., Geburek, F. Equine Lameness Detection and Monitoring with an Instrumented Hoof Boot.

In: Proceedings of the 5th Scientific Meeting of the European College of Veterinary Sports Medicine and Rehabilitation, ECVSMR; Cordoba, Spain, October 16-18, 2024; in print

 

Geburek, F., Jung, K., Keller, J. Bewegungsanalyse mit instrumentierten Hufschuhen - was ist möglich?

In: Tagungsband des DVG-Vet-Congress 2024 - 7. Internationaler Kongress zur Pferdemedizin / Tagung der DVG-Fachgruppe Pferdekrankheiten, 1.- 2. November 2024, Berlin, Verlag der DVG Service GmbH, Gießen, ISBN 978-3-86345-736-5, S. 44-46

 

Geburek, F. Objective gait analysis with instrumented hoof boots: What is possible?

In: British Equine Veterinary Association (BEVA) Congress 2025, September 10- 13, 2025, Birmingham, United Kingdom, p. 161

 

Keller, J. Validierung und klinische Anwendung eines mit Druck- und Lagesensoren ausgestatteten Hufschuhs bei Pferden. Dissertation, Tierärztl. Hochsch. Hannover, 2025; Cuvillier Verlag, Göttingen.

 

Geburek, F., Kopf, A.-K., Jung, K., Hassenstein, M., Keller, J. In-vivo validation of an instrumented hoof boot for gait analysis equipped with pressure sensors and an inertial measurement unit.

In: Proceedings of the 71st AAEP Annual Convention, December 6-10, 2025; American Association of Equine Practitioners, Denver, CO, USA, p. 90-91

Project Details:
The objective of this prospective randomised clinical trial is to evaluate the occurrence of post-operative ileus as well as the short- and long-term outcome with the use of two different suture patterns for jejunojejunal anastomosis. Furthermore, histomorphology and several markers for inflammation, cell death, the enteric nervous system and stem cells will be evaluated in intestinal samples taken from the resection margins. In cases necessitating anastomotic revision or euthanasia, this (immuno-)histological examination will also be performed on the anastomosis itself. Determining the expression and distribution of these markers will enable the characterisation of this tissue prior to and following anastomosis and possibly identify causes for post-operative ileus or anastomotic failure.

Project Details:
Flexural deformities are common in foals. Conservative therapy by systemic administration of oxytetracycline (OTC) is often effective, however, it is associated with risks and disadvantages. The aim of the project is to test the potential effect of pharmacological alternatives to OTC on tendon tissue ex vivo.

Results:

Part A) In this study, the influence of substances with potentially similar

mechanisms of action, however without antimicrobial properties, on viability and proliferation of

juvenile myofibroblasts was assessed in vitro. Myofibroblasts from forelimb superficial digital flexor

tendons and accessory ligaments of the deep digital flexor tendon from 6 foals, euthanized for reasons

unrelated to this study, were cultured and characterized. The myofibroblasts were incubated with

oxytetracycline, the MMP-inhibitors incyclinide, ilomastat, aprotinin, pentoxifylline, the lathyrogenic

agent β-aminopropionitrile fumarate and Dulbecco?s modified eagle medium as control. Colorimetric

cell viability (MTS) and crystal violet assays assessed their viability and proliferation capacities. The

morphology and immunohistochemistry profile of the cultured cells was consistent with tendon and

ligament myofibroblasts. All test substances were biocompatible, shown by the absence of significant

differences with cells incubated with medium, demonstrating the absence of cytotoxic or antiproliferative effect on juvenile myofibroblasts in the tested concentrations in this preliminary study.

https://doi.org/10.1038/s41598-025-00311-z

Funding: MWK, 540.878 EUR

Project Details:
Comprehensive characterization of the hormone profiles of the HPG axis in male and female Long COVID patients, hormone panels, hormone measurements and statistical evaluations.
Creation of expression profiles of sex hormones and
sex hormone receptors in the plasma, lungs, reproductive organs and brains of male and female hamsters.

Results:

https://www.umg.eu/forschung/corona/cofoni/