Research

Funding: Niedersächsisches Ministerium für Wissenschaft und Kultur , 50.000 EUR

Project Details:
Within a blended learning event, content on interprofessionalism and teamwork is delivered across professional groups via self-study units in TiHoMoodle, in video conferences and in live events in the communication room in the Clinical Skills Lab. With the help of actors, relevant aspects of communication between veterinarians and veterinary assistants as well as animal owners are simulated and practiced so that the foundation can be laid for effective and harmonious collaboration in veterinary practice.

Results:

OER-Beitrag: https://www.twillo.de/edu-sharing/components/collections?id=784dd823-e75e-48ed-a8c3-6de63136b02b

https://doi.org/10.3390/ani14020229

Funding: Frauenhofer ITEM, 278.466 EUR

Project Details:
Frauenhofer ITEM-Therapeutic treatment against SARS-CoV-2 with inhaled VHH from BiondVax - Efficacy program in hamsters

Funding: MWK, 22.000 EUR

Project Details:
The importance of the highly conserved protein Nsp15 for the infection of cells by coronaviruses has been studied in detail in recent years. As an endonuclease, it rapidly recognizes and degrades both single-stranded (ssRNA) and double-stranded RNA (dsRNA). In addition, evidence from animal models suggests immunomodulatory properties of Nsp15 during early viral infection, leading to a delayed immune response. This process is ultimately involved in the development of acute respiratory failure, which is the main cause of COVID-19 mortality. As part of this funding program, we plan to develop new inhibitors of this protein through structure-guided design. Promising compounds will be characterized with respect to their ADME and physicochemical properties in order to obtain orally bioavailable compounds for the treatment of future viral pandemics.

Results:

https://www.umg.eu/forschung/corona/cofoni/

Funding: MWK, 220.890 EUR

Project Details:
CRISPR/Cas13-mediated antiviral therapy against SARS-CoV-2 and its variants: a novel technology to target viral genome

Results:

https://www.umg.eu/forschung/corona/cofoni/

Funding: BLE/ Unterauftrag FLI, 78.300 EUR

Project Details:
Pig production has grown dramatically worldwide over the last 20 years, leading to increased herd sizes
with weekly production cycles of piglets. In the meantime, the dynamics of infections with swine influenza A
viruses (swIAV) have changed, from epizootic acute respiratory
outbreaks to a self-sustaining infected status of affected herds. Moreover, the genetic and antigenic
diversity of swIAVs in European pig populations has dramatically increased in recent years. Thus, novel
reassortants and antigenic variants have emerged regionally, that can escape control strategies based on
vaccines licensed for use in Europe. The self-sustaining forms of swine influenza (SI) in large holdings
adversely affect animal health and welfare, and prompts the excessive use of antibiotics when swIAV is
associated with other respiratory pathogens in the porcine respiratory disease complex (PRDC), resulting in
severe economic losses. There is an urgent need to increase knowledge of within-herd virus dynamics and
evolution in order to design intervention and prevention measures to limit swIAV persistence in intensive
herds and counteract continuous production losses and emergence of new swIAVs.The objectives of the
research project PIGIE are:
- to define the epidemiological and economic factors that drive the prevalence and dynamics of swIAV in
large pig herds,
- to evaluate the impact that swIAV enzootic infections have on animal welfare, production parameters and
economic productivity,
- to study the genetic and antigenic diversity of swIAV in Europe,
- to identify the host-pathogen factors that would foster swIAV evolution,
- to provide a better understanding of long-lasting and protective immunological memory responses
developed in the infected hosts,
- to identify mitigation points in continuously infected herds,
- to implement and evaluate control strategies that would help to counter sustained infections in closed
intensive herds.

Cooperation Partners:

FLI, Riems

Funding: Frauenhofer ITEM, 331.375 EUR

Project Details:
Therapeutic treatment against SARS-CoV-2 with inhaled VHH from BiondVax - Efficacy Program in Hamsters

Funding: DZIF, BMBF, 65.000 EUR

Project Details:
In May 2022, an unprecedented multi-country outbreak of monkeypox affecting Europe, North America, the Middle East, and Australia was recognized. Monkeypox (MPX) is a re-emerging, zoonotic, and potentially life-threatening viral disease caused by monkeypox virus (MPXV), an orthopoxvirus endemic in Central and West Africa. As of June 28th 2022, a total of 4119 confirmed cases have been reported worldwide (ECDC), including 676 confirmed cases in Germany (RKI). This is the first time that chains of human-to-human MPXV transmission are documented without epidemiological links to West or Central Africa. At present, most cases are detected in young men, self-identifying as men who have sex with men, and associated with mild clinical symptoms. However, MPXV may cause severe disease in risk groups of the population (young children, pregnant women, immunosuppressed persons), and a spread of MPX into the general population is to be expected as most of the younger population (<50-60 years of age) lack virus specific immunity because of the end of smallpox vaccination programmes in the 1980ties. Thus there is a risk of an extended period of MPX circulating also outside of Africa, and ongoing efforts in the public health response must interrupt MPX transmission chains and clear MPXV from its new environment as soon as possible. Current countermeasures include the use of a licensed smallpox vaccine (IMVANEX) based on Modified Vaccinia virus Ankara (MVA) and a licensed drug against smallpox (Tecovirimat). STIKO recommends vaccination with IMVANEX as post-exposure prophylaxis for contacts of MPX patients and for persons at high risk of exposure.
The aim of this application is to support this counteraction measures against the MPX outbreak by preparing for the development and clinical use of the DZIF MVA-SARS-2-S candidate vector vaccine as bivalent vaccine against MPX and COVID-19 including the establishment of a preclinical model for immunogenicity and efficacy testing.

Cooperation Partners:

Prof. Dr. Marylyn Addo, UKE Hamburg

Prof. Dr. Gerd Sutter, LMU München

Funding: Ministerium für Energie­wende, Klimaschutz, Umwelt und Natur, 98.692 EUR

Project Details:
The two invasive fungi Batrachochytrium dendrobatidis (hereafter referred to as Bd) and B. salamandrivorans (hereafter referred to as Bsal) originate from Asia. Bd is known to infect more than 700 species of amphibians of all three clades (caecilians, salamanders, frogs). A Bd infection can lead to clinical disease and ultimate death of affected animals. Clinical disease though, like the characteristic skin lesions, are not present in all cases. The so-called chytridiomycosis is therefore versatile and difficult to predict. Bsal was first discovered in Europe in the Netherlands in 2013, where the pathogen has been associated with a decline of more than 95% of the fire salamander (Salamandra salamandra) population since 2010. Since then, outside of Asia, it was found in several European countries, including Germany. The overall aim of the project is to provide better knowledge of the actual distribution of both invasive fungi Bd and Bsal in Schleswig-Holstein over a period of two years. Therefore, different amphibian species sharing the same habitats in Schleswig-Holstein are targeted. Sampling uses sterile skin swabs, which represents an amphibian friendly method. Right after sampling, animals are released. Ideally, the outcome of this pilote study will give first ideas of regional, seasonal and species specific characteristics of the pathogens and will thus shape future research in this field.

The following questions will be addressed:
-Is there a direct link between the detection of Bd and Bsal and clinical symptoms or even an increased mortality?
-Which species typically show symptoms of clinical disease? Which species
are infected without symptoms (subclinically), function as asymptomatic carriers or as a reservoir?
-Are there regional differences in the prevalence of the pathogens?

Due to the critical situation of Schleswig-Holsteins amphibians, it is of utmost concern to apply management measures to protect amphibian populations of additional threats like infectious diseases. Additionally, the outcome of the project will therefore directly serve conservation efforts and help improve reintroduction efforts and management measures for amphibians.

Results:

Abschlussbericht an das Ministerium für Energiewende, Klimaschutz, Umwelt und Natur

https://www.tiho-hannover.de/fileadmin/57_79_terr_aqua_Wildtierforschung/79_Buesum/downloads/Berichte/2023_Amphibien_Abschlussbericht_MEKUN.pdf

Cooperation Partners:

Christoph Leineweber, Rachel Marschang (LABOKLIN GmbH)

97688 Bad Kissingen

Christian Winkler (Arbeitskreis Herpetofauna der FÖAG)

24582 Bordesholm

Patrick Pohlmann (Gesellschaft für Freilandökologie und Naturschutzplanung)

24145 Kiel Wellsee

Arne Drews (Landesamt für Landwirtschaft, Umwelt und ländliche Räume)

24220 Flintbeck

Funding: Umweltbundesamt, 264.210 EUR

Project Details:
Under the Act on the Implementation of the Protocol on Environmental Protection to the Antarctic Treaty (AUG), the Federal Environment Agency (UBA) is the national licensing authority for all activities in Antarctica that are organised from or emanate from Germany. As part of the licensing procedure under the AUG, an environmental assessment must be carried out by UBA as the implementing authority for all notified activities in the area covered by the Antarctic Treaty.
Seismic surveys using airguns are carried out to reliably determine the topography and geological composition of the seabed. These devices emit acoustic signals with high sound pressure levels. So far, the data basis is insufficient to assess possible impacts on marine fauna.
The aim of this project is to assess the effects of a geoscientific, seismic survey with airguns during the expedition "WAIS-BELL" (PS 134) in 2022/23 on cetaceans in the Antarctic. Here a behavioural response study will be carried out, which will include a multidisciplinary approach combining visual recording methods and passive acoustic monitoring (PAM).
The project aims to generate data on the distribution, habitat use and possible behavioural responses of cetaceans in their Antarctic feeding area in an undisturbed and acoustically disturbed situation. The work will be carried out during seismic surveys in the Amundsen and Bellingshausen Seas and will be carried out in close cooperation between TiHo, JASCO and the AWI geophysics group in their survey programme.
The noise exposure and vocalisations of cetaceans are recorded using an autonomous acoustic recording system (AMAR), which allows the identification, localisation and tracking of vocalizing individuals. Visual recording methods (helicopter, autonomous drone, possibly bridge or crow's nest of FS Polarstern) will be used to collect data on the distribution and behavioural responses during the seismic survey as well as habitat use of the cetaceans during transits to and from the study area.
The results will contribute significantly to assessing the potential effects of seismic surveys with airguns on whales and provide important information for the management of anthropogenic activities in the Antarctic Treaty Area.

Results:

The project aimed at assessing the effects of seismic airgun surveys during the PS134 (WAIS-BELL) expedition on Antarctic cetaceans. A multidisciplinary monitoring concept combining visual observations and passive acoustic methods was developed to investigate behavioural responses, avoidance distances, and potential masking of whale vocalisations.

Ship-based observations by marine mammal observers (MMOs) and a validation team proved to be the most reliable mitigation measure. A comparison showed that teams of two MMOs achieved nearly the same detection performance as teams of three. Based on all shipboard sightings, a median avoidance radius of 3.4 km was determined when airguns were active, compared to 1.2 km when they were inactive. Limitations included weather, darkness, observer variability, and blind spots behind the vessel. The infrared (IR) camera provided some additional detections, but was constrained by technical issues, humidity, and a large blind sector. Nevertheless, system upgrades are recommended because of its utility during darkness. Helicopter aerial surveys significantly expanded the observation radius (up to 90 km) and gave insights into species distribution, but are not suitable for real-time mitigation. After initial technical issues, the drone showed potential for future real-time monitoring, though it could not yet be fully evaluated.

 

An Autonomous Long-Term Observatory (ALTO) system with an integrated AMAR was intended to record vocal activity before, during, and after seismic operations, but produced no data due to a technical malfunction, despite the general suitability of this technology. The PAM system QuietSea™ integrated into the streamer generated mostly false alarms. Among many detected events, only one single automatic detection was confirmed as a real baleen whale vocalisation. Possible reasons include masking by vessel noise, the bubble curtain behind the ship, and insufficient calibration or hardware issues.

No single method provided complete and reliable marine mammal monitoring under all conditions. Ship-based visual monitoring currently remains the most effective mitigation tool. IR systems, drones, and acoustic technologies require technical improvements, but could become valuable complementary tools, particularly in darkness, poor weather, or for underwater detection. A robust mitigation and protection strategy will require a combination of optimised monitoring approaches.

Cooperation Partners:

JASCO Applied Sciences

Prof. Rochelle Constantine und Tane van der Boon (MAUI63 project New Zealand)

Dr. Brandon Southall (Southall Environmental Associates, Inc., SEA)

Funding: Deutsche Wild Tier Stiftung, Hamburg, 8.415 EUR

Project Details:
The harbour porpoise (Phocoena phocoena) is the only species found in German waters. This species is protected as an Annex II and IV species under the Habitats Directives, among others. Harbour porpoises inhabit coastal waters, which means that their distribution largely overlaps anthropogenic activities. The resulting pressures on the harbour porpoise range from noise pollution and competition for prey fish to accidental bycatch in set net fisheries. The area of the Eckernförde Bay in the western German Baltic Sea is characterised by a high degree of anthropogenic use and thus has a high potential for disturbance of harbour porpoises. Set net fishing is widespread in this area and is the fishing method with the highest incidental bycatch. The area around Eckernförde also has a high incidence of shipping, as it is a rather narrow bay from which many fishing vessels leave, a harbour and training area for military activities, and a high area of tourist activities is situated here.
In order to develop effective measures for the protection of harbour porpoises, it is first necessary to investigate the habitat use of harbour porpoises in this area. In the western part of the German Baltic Sea, acoustic monitoring has been carried out by ITAW since spring 2021, but there is a gap in the area of the Eckernförde Bay. Within the framework of this research project, a monitoring station is to be deployed in the Eckernförde Bay that can meaningfully close this gap. The monitoring station will be equipped with a CPOD in spring, which will be replaced after about 3 months in order to collect data on harbour porpoise occurrence for a total of about 6 months. Underwater sound will be recorded simultaneously allowing for correlating harbour porpoise presence with the occurrence of anthropogenic noise.

Results:

In 2021, continuous acoustic monitoring of porpoises was launched for the first time in the Schleswig-Holstein section of the Baltic Sea, between the Flensburg Fjord and Eckernförde Bay. In 2023, a further monitoring station was added, extending the monitoring network southwards. A total of five monitoring stations (Holnis, Bredgrund, Schleisand, Damp and Waabs) are in operation, equipped with click detectors (C-PODs) to record the echolocation sounds (clicks) of porpoises. The monitoring has provided acoustic data on the occurrence and seasonal presence of porpoises.

In addition to genuine porpoise clicks, an increasing number of artificial porpoise clicks have been recorded from acoustic warning devices. These warning devices are known as Porpoise Alerting Devices (PALs) and are used in gillnet fishing to warn porpoises of the presence of nets by emitting artificial clicks resembling those of porpoises, thereby reducing bycatch.

PAL signals accounted for only a relatively small proportion of the total number of clicks detected. However, their significance lies less in the absolute number of clicks than in their prolonged presence over many days. An exceptionally high duration of PAL activity was observed in the study area of the western Baltic Sea. At the Bredgrund and Waabs stations, over 100 and in some cases nearly 200 days with active PALs were recorded in both 2023 and 2024. Given this high duration of activity, it must be assumed that the habitat is strongly influenced by human activity. It should be noted that C-PODs have a detection range of less than 200 m. Possible large-scale deterrent effects or behavioural changes in porpoises outside this radius cannot be captured by the available data. The actual influence of PALs could therefore be significantly greater in spatial terms than is apparent from the monitoring. The analysis of the C-POD PAL array clearly showed that porpoises emit significantly fewer clicks during active PAL phases. This suggests avoidance responses or a suppression of echolocation activity. The results thus contradict the assumption that PALs have exclusively a neutral warning function, and instead point to potentially deterrent effects.

In addition to the PAL issue, the presence of porpoises was investigated during the Bundeswehr’s blasting campaigns between 2022 and 2024 at the Schleisand and Damp monitoring stations near the Schönhagen study area. The results show that porpoise sightings were reduced during the blasting and only resumed some time after a blast. The period following the explosions may depend on the explosive charge.

The results of porpoise monitoring in the western Baltic Sea show that PALs not only interfere with acoustic monitoring from a technical perspective, but also cause measurable changes in porpoise behaviour. Areas with a long-term presence of PALs must therefore be classified as habitats subject to significant anthropogenic influence.

The combination of continuous monitoring, PAL filtering, model-based analysis and the consideration of acute disturbance events such as blasting provides an important basis for management and conservation measures under the Marine Strategy Framework Directive (MSFD). Continuation and further development of the monitoring programme are necessary to better assess long-term trends and cumulative effects.