Research

Funding: DFG, 535.000 EUR

Project Details:
When dry preservation of gametes and/or genetic material were possible without loss of fertilizing potential, this can be implemented for securing genetic resources and would transform biobank facilities and the breeding industry. This would facilitate low-cost room temperature storage easing off-the-shelf availability and transport. Moreover, dry preservation methods for biologics can be implemented in underdeveloped countries, remote locations, and non-laboratory settings. Without taking protective measures, biomolecules in mammalian cells are subject to (irreversible) conformational changes during drying and specimens are prone to chemical degradation during storage, impairing their functions. Numerous attempts have been pursued to preserve cell viability and functionality in the dried state, mostly inspired by nature’s way to survive drying in a state of suspended animation referred to as anhydrobiosis. However, only limited successes have been reported in keeping ordinary mammalian cells viable after drying and subsequent rehydration. Attempts focused on the introduction of specific disaccharides (i.e., trehalose, sucrose), stress proteins, and membrane modification strategies, which play a role in acquiring desiccation tolerance in nature. However, the complex orchestrated cellular adaptation mechanisms of anhydrobiotic organisms are not likely to be mimicked in cells that are not naturally resistant to dehydration. Dried mammalian cells may retain their structure and specific functional properties. For example, dried sperm and somatic cells can be injected into (enucleated) oocytes for the production of offspring. However, under ambient conditions, biomolecules in dried cells are susceptible to rapid degradation during storage. Major factors impairing storage stability of biomolecular structures in a dried cellular environment are the presence of reactive molecules, environmental conditions, and inherent susceptibility for damage (e.g., degree of lipid saturation and chromatin condensation). We propose a different approach for long-term room temperature preservation of genetic resources; not focusing on recovering fully functional cells but by generating ‘artificial’ cell-like nuclei-containing structures. We plan to do this by (1) making ‘ghost cells’ from sperm to remove damaging reactive molecules normally present in the cells, while keeping organelles and cytoskeletal elements inside, and (2) by making demembranated sperm encapsulated in giant liposomes. In both cases, damaging reactive molecules in cells are removed, while protective molecules can be easily introduced.

Funding: Niedersächsisches Ministerium für Wissenschaft und Kultur (MWK) ZERN - Zukunft Ernährung Niedersachsen, 365.144 EUR

Project Details:
This project is carried out at DIL e.V., Quakenbrück.

The project provides evidence-based insights into the sensory quality, microbiological safety and potential health effects of raw milk kefir. The results identify conditions under which raw milk kefir can be a viable option for regional direct marketing. A practical guideline supports dairy farms in quality-assured production and marketing.

Cooperation Partners:

Deutsches Institut für Lebensmitteltechnik (DIL), Quakenbrück, Abteilung Biochemie der Ernährung

Georg-August-Universität Göttingen, Fakultät für Agrarwissenschaften, Labor für sensorische Analysen und Konsumentenforschung, Department für Agrarökonomie und Rurale Entwicklung

Project Details:
The aim of this follow-up project is to investigate the contribution of structural genomic variation to the genetic architecture of growth in the pig model. The focus is on the identification and characterization of structural variants using long-read sequencing and their regulatory effects in non-coding genomic regions. Analyses of animals with divergent growth phenotypes will be used to detect growth-associated structural variants and selection signatures. These variants will be evaluated with respect to their location in regulatory sequences, putative enhancers, and chromatin domains, and linked to gene expression data from growth plate tissue. The objective is to identify functionally relevant non-coding variation affecting growth-regulatory processes.

Cooperation Partners:

Prof. Tim Kacprowski, Leiter Abteilung Data Science in Biomedicine, Peter L. Reichertz Institut für Medizinische Informatik der TU Braunschweig und der Medizinischen Hochschule Hannover

Funding: Niedersächsisches Ministerium für Ernährung, Landwirtschaft und Verbraucherschutz, 7.500 EUR

Project Details:
The objectives of the project include determining the level of genetic diversity, the genetically effective population sizes, and the extent of gene flow, as well as the association between microsatellites and SNPs.

Cooperation Partners:

Kooperation zwischen der Georg-August-Universität Göttingen (GAUG, Abteilung Wildtierwissenschaften), der Justus-Liebig-Universität Gießen (JLU, Arbeitskreis Wildbiologie, AG Reiner) und der Stiftung Tierärztliche Hochschule Hannover (ITAW, Institut für Terrestrische und Aquatische Wildtierforschung) sowie dem Landesjagdverband Niedersachsen (LJN)

Funding: Bundesministerium für Wirtschaft und Energie (BMWE) im Rahmen der Industriellen Gemeinschaftsforschung (IGF) aufgrund eines Beschlusses des Deutschen Bundestages, 524.935 EUR

Project Details:
The "SweetGut" project investigates the effects of non-nutritive sweeteners (NNS) on the human gut microbiome, intestinal barrier function and inflammation-related processes. Both individual sweeteners and commonly used combinations in sweetened dairy products are examined.
Using in vitro cell models and ex vivo fecal models, potential adverse physiological effects such as microbial dysbiosis and proinflammatory responses are analyzed. In addition, the project evaluates whether functional oligosaccharides (e.g. inulin, GOS, FOS) can mitigate or compensate for these effects.
The overall aim is to provide a scientific basis for safe, health-oriented reformulation of sugar-reduced dairy products and to support consumer protection.

Cooperation Partners:

Deutsches Institut für Lebensmitteltechnik (DIL), Quakenbrück, Abteilung Biochemie der Ernährung; Abteilung Biotechnologie

Max-Rubner-Institut (MRI), Bundesforschungsinstitut für Ernährung und Lebensmittel, Institut für Mikrobiologie und Biotechnologie, Kiel

Funding: Niedersächsisches Ministerium für Wissenschaft und Kultur (MWK) ZERN - Zukunft Ernährung Niedersachsen, 30.000 EUR

Project Details:
The project is carried out on DIL e.V., Quakenbrück.
The project "HIDDEN PEATENTIAL! - Exploring the Hidden Potential of Plant-Based Proteins" aims to communicate scientifically sound knowledge on sustainable nutrition and alternative protein sources, with a particular focus on plant-based proteins derived from legumes such as peas.
Using a Citizen Lab outreach format, two interactive public events will be held in pedestrian zones in Osnabrück and Quakenbrück. Through tastings, hands-on experiments, playful learning formats and direct dialogue with scientists, citizens are invited to engage with topics such as sensory properties of plant proteins, amino acid composition, techno-functional characteristics (e.g. foaming properties of aquafaba), and environmental impacts of different protein sources.
The project contributes to public science communication within the ZERN network and serves as a pilot for transferable outreach formats supporting informed and reflective decision-making in the field of sustainable nutrition.

Cooperation Partners:

Deutsches Institut für Lebensmitteltechnik (DIL), Quakenbrück, Abteilung Biochemie der Ernährung;

Universität Osnabrück

Funding: Bundesanstalt für Landwirtschaft und Ernährung (Bundesprogramm ökologischer Landbau), 231.160 EUR

Project Details:
The overarching objective of the project is to improve the health of suckling and weaned piglets under organic farming conditions in free-farrowing systems and piglet rearing systems through optimized housing and feeding strategies, and to identify approaches for reducing piglet mortality. In addition to adapted housing and nutrition, an improved, farm-specific health and hygiene management system is intended to contribute substantially to stable herd health in organic production systems. Furthermore, the project aims to close existing knowledge gaps and generate new scientific insights for organic sow husbandry and piglet production through the investigation and evaluation of multiple research questions.

Cooperation Partners:

Landwirtschaftskammer Nordrhein-Westfalen

Georg-August Universität Göttingen

Universität Kassel

Funding: DFG (VIPER GRK)

Project Details:
Characterizing the impact of morbilliviruses upon innate responses and the integrity of respiratory epithelial cells.
-Generation and characterization of 3D-culture systems of different wildlife carnivore species
-Analyses of morbillivirus infections upon respiratory tract cells of wild carnivores in vivo and ex vivo

Funding: DFG (VIPER GRK)

Project Details:
Hypothesis:
Viral genomic sequences obtained from archived canine formalin-fixed, paraffin embedded tissue of the central nervous system and will allow establishing a cause-consequence relationship between the detected known and unknown viral pathogen and pathological findings.
Aim and objectives:
The general aim of the project is further identify the viral cause of canine diseases of the central nervous system.
-By using conventional histology and immunochistochemistry of archived tissues from the last 6 decades to identify cases of non-supprative encephalitis of unknown origin
-Suspected virus infection will be further substantiated by applying double-stranded RNA or selected interferon stimulated genes specific antibodies and a probe specific for interferon β.
-Followed by ribonucleic acid extraction from the selected FFPE tissues, RT-qPCR using pan-genus primer and/or NGS. Alignment to reference genomes available in databases will be used to detect known viral pathogens and related unknown viruses
-Investigation of the pathogenesis of the discovered virus by studying cell tropism and the distribution of the virus in the host organism using in situ hybridization for the distribution of the pathogen

Cooperation Partners:

Institut für Virusdiagnostik, Friedrich Löffler Institut

Funding: Gefördert mit Mitteln aus zukunft.niedersachsen, 612/ZERN 7897049-TiHo - Open Call, 301.520 EUR

Project Details:
Animal welfare and animal health are, among other factors, substantially influenced by the barn climate. Establishing an optimal barn climate is a complex task, and additional challenges arise from changes in the external climate.
In this project, conducted in collaboration with partners from academia and industry, barn climate parameters and floor temperatures in a forward-looking, animal-friendly housing system are adjusted on the basis of measurements, AI (artificial intelligence)-supported behavioral observations, and accompanying hygiene monitoring. The aim is to ensure that pigs in the rearing and fattening phases make optimal use of pen structures. At the same time, the project investigates from which point onward barn climate control based on the use of enthalpy values becomes meaningful. Furthermore, based on the collected data, heat transfer processes will be represented within a pen model in order to better understand their effects on the microclimate in individual pens.

Cooperation Partners:

Prof. Dr.-Ing. Markus Richter, Robin Kahlfeld, M. Sc,

Institut für Thermodynamik, Fakultät für Maschinenbau,

Leibniz Universität Hannover

Assoziierte Partner:

Big Dutchman AG

H. Bröring GmbH & Co. KG

VetVise GmbH