Research

Funding: The International Human Frontier Science Program Organization (HFSP), 1.106.000 EUR

Project Details:
Insect cell membranes differ from mammalian membranes by deformability, lipid content and distribution. Enveloped insect-borne viruses require intimate interactions with cellular
membranes to enter cells, replicate their genomes in cells and bud from cells. Despite fundamental biophysical differences between insect and mammalian membranes, viruses can productively infect cells from both phyla. Decades of studies on insect-borne viruses have not addressed the machinery of insect membrane deformation and its exploitation by viruses. Working at the interface of insect genetics, biophysics and infection biology, our newly formed team has assembled innovative technologies to break through this barrier in the field. Through the combined expertise of the PIs, we will: 1. Employ gene editing libraries in insect cells to identify host factors steering virus - membrane interactions; 2. Image membrane deformation at high resolution; 3. Assess the impact of membrane deformation on permissiveness to insectborne viruses. These studies will provide insight into mechanisms driving insect membrane shape and revolutionize our understanding of virus adaptation across fundamentally different
species.

Cooperation Partners:

Dr. Nicholas Ariotti, Institute for Molecular Bioscience, Australia

Dr. Norbert Perrimon, Harvard Medical School, USA

Funding: DFG, 498.400 EUR

Project Details:
The equine recurrent uveitis (ERU) is the main reason for blindness in horses, and 10% of the european horses are affected. Significantly, ERU is an important available model for human autoimmune uveitis as the clinical and immune-pathological characteristics are similar. As a cause for ERU, various hypotheses are described in the literature. On the one hand, infections with Leptospira interrogans are discussed as well as other bacterial, parasitic or viral infections. Independent of the initiating cause, there are numerous indications that ERU is an immune-mediated disease. In the body after the control of the primary infection, an overreaction of the immune system occurs in response to activated immune cells. In addition to lymphocytes, these immune cells can also be neutrophil granulocytes. Among various antimicrobial functions, neutrophils have been shown to release neutrophil extracellular traps (NETs). NETs are extracellular DNA fibers with associated proteases and antimicrobial peptides which are released by activated cells. NETs mediate entrap of invading pathogens, but when massively released may contribute to detrimental autoimmune reactions of the host.
The research project presented here is intended to provide essential insights for the involvement of NETs in the pathogenesis of ERU. In the first phase of our study, our findings indicate an involvement of NETs in the pathogenesis of ERU with detrimental effects. In short, NETs correlate to disease severity, seem to damage the blood retinal cells, and induce autoantibodies against NET proteins. We hypothesize, that those autoantibodies might shield the NETs from degradation by DNases, resulting in their persistence and potentially in new disease episodes. Thus, in the current project we aim to characterize if and how the phase of disease (non-active quiescent phase versus acute inflammatory phase) influences cellular involvement and extracellular DNA release. Based on our previous data, we further hypothesize that different cell types besides neutrophils play a role in extracellular DNA release during the progression of ERU, which enables crosstalk between various immune cells. Therefore, we will study the release of ETs by other immune cells besides neutrophils and its interplay with adaptive immune cells. Finally, since the use of gentamicin in the treatment of ERU patients is well established, the aim is to determine the influence of gentamicin in varying concentrations and along with distinct stimuli on equine immune cells, focusing on ET release.
 

Funding: Niedersächsisches Ministerium für Wissenschaft und Kultur (MWK), 324.000 EUR

Project Details:
The aim of this subproject is the further development and optimization of a 3D lung epithelial cell culture model
for the investigation of viral and bacterial infections and the effect of immunomodulatory
immunomodulatory substances on the course of infection and cell biochemical processes. An increased
complexity of the model system using physiological or pathophysiological oxygen
conditions and microfluidic systems enables the in vitro system to be approximated to the in vivo situation and
to the in vivo situation and will help to reduce the number of test animals in the long term. The
complexity of the infection model is reduced by physiologically relevant oxygen conditions (defined
tissue-specific hypoxic conditions) and by 3D co-cultivation of human or animal pulmonary
or animal pulmonary epithelial cells and neutrophil granulocytes. In addition
the use of primary cells and induced pluripotent stem cells should provide long-term optimization.

Funding: Bundesministerium für Bildung und Forschung, 625.000 EUR

Project Details:
As long as animal experiments cannot be completely replaced, the question will arise on which animals research should take place. In this project, an interdisciplinary working group wants to investigate the ELSA-relevant question, according to which criteria the selection of laboratory animal models takes place or should take place. The aim of the enterprise is to determine the manifold reasons for the choice of certain laboratory animals; the process is interdisciplinary in the full sense of the word and put the different rationalities into a well-founded correlation. The question condenses several open and important issues concerning the treatment of animals. The answer is to be examined in each of the disciplines involved, between them and finally in their association, whether it is possible to establish a coherent catalog of criteria that also creates clarity for the practice of animal experimentation, both for everyday research and at the interface to legislation and enforcement. This is important against the background of rapidly changing social value judgements about animals, which also lead to permanent tensions here. In this project we want to investigate the ELSA-relevant question of the criteria for the selection of laboratory animal models. We follow an interdisciplinary approach, which is represented by the following cooperation partners: Its members are: Prof. Dr. Peter Kunzmann (ethics), Prof. Dr. Bernhard Hiebl (laboratory animal science), Prof. Dr. Maren von Köckritz-Blickwede (infection research) (all TiHo- Hannover) and the legal scientist Prof. Dr. Dr. Tade Spranger (University of Bonn).

Cooperation Partners:

Tade Spranger, Rechts- und Staatswissenschaftliche Fakultät, Bonn

Funding: BUND Landesverband Nidersachesen e.V., 37.018 EUR

Project Details:
Underwater noise from ship traffic, the construction of facilities in the water and on the coast or the explosion of ammunition in the sea is identified as a threat by various national and international expert assessments and has negative effects on marine animals in the Wadden Sea - especially on marine mammals, including porpoises. Many projects on the effects of noise pollution on marine mammals underwater are already being carried out at the ITAW. The ITAW is particularly concerned with the effects of underwater noise on harbor porpoises, with a particular focus on ship traffic, offshore constructions, and unexploded ordnance contaminated sites (e.g. BfN-UWE2, BMBF-COASTALFUTURE, EU project SATURN). In this joint project with BUND, ITAW will provide support with recommendations for measures to reduce the effects of underwater noise on porpoises, development of voluntary rules of conduct, participation in panel discussions and materials for exhibitions. Different target groups should be made aware of the need to reduce underwater noise through different offers (exhibitions, lectures in schools, raising awareness among recreational boat owners). At the same time, recommendations for concrete measures to reduce underwater noise pollution are being developed.

Funding: Niedersächsisches Ministerium für Wissenschaft und Kultur, 178.000 EUR

Project Details:
Measure 1 consists of work package A, which is part of e-learning service. The use of the blended learning format in veterinary teaching is to be promoted. A further focus will be placed on strengthening the (e-)didactic skills of lecturers and the digital skills of students, for which training courses will be created for these target groups.

Measure 1 also includes work package B, which is located in the Clinical Skills Lab. The establishment of a surgical logbook is intended to strengthen students' surgical skills. To support the implementation of the surgical logbook, a training concept for teaching surgical skills is being developed.

Funding: Landesbetrieb für Küstenschutz, Nationalpark und Meeresschutz S-H (LKN), 42.025 EUR

Project Details:
Harbor porpoises are representatives of the top mammalian predators in the North Sea and Wadden Sea. With the amendment of the National Park Act in 1999, part of the national park was explicitly dedicated to the protection of harbor porpoises, as a high density of mother-calf groups was found there. As part of the reorganization of the Federal-Länder Marine Programme (BLMP), a programme for the joint monitoring of marine mammals was agreed in January 2011, which meets the requirements of monitoring in accordance with the relevant European directives and international conventions. The Schleswig-Holstein National Park Administration (NPV) within the Schleswig-Holstein State Agency for Coastal Protection, National Park and Marine Conservation (LKN) has the task of organizing, implementing and financing the acoustic monitoring of harbour porpoises in this programme. The acoustic surveys for the NPV are carried out by the Institute for Terrestrial and Aquatic Wildlife Research (ITAW) as part of the joint marine mammal monitoring program under the BLMP. For this purpose, measuring stations are operated at five defined locations in the Schleswig-Holstein Wadden Sea (Lister Tief, Westerland, Rochelsteert, Meldorfer Bucht and Outer Elbe). The monitoring stations are equipped with click detectors (C-PODs), which record the echolocation activity of harbor porpoises. The C-PODs are serviced and read out at regular intervals. The data obtained in this way is evaluated against the background of the optimization of the acoustic monitoring as well as necessary extensions for a permanent operation of the measuring stations. The data from this long-term study will provide information on the possible rhythm and tidal dependency of the harbor porpoise detections throughout the day and over the course of a year.

Funding: Ministerium für Energiewende, Landwirtschaft, Umwelt, Natur und Digitalisierung , 350.135 EUR

Project Details:
After unexpected problems occurred during the data collection in the field, an objectively evaluable data basis will be created, aming at evaluation of the acoustic deterrence system. In addition to the working task, further analyses will be carried out to assess the management measures developed and implemented to date outside the public swimming area, which include, for example, the previously established protection zones or the panoramic path. In order to ensure an effective management, regular evaluation and possible optimization is essential. The counting data collected on Helgoland by Jordsand / Dünen-Ranger represents an extremely important data basis for this. Based on an extremely important data basis for this juvenils, a corresponding evaluation of the effectiveness of the measures will be carried out.
In order to investigate the temporal and spatial use of areas by grey seals in the North Sea in more detail and also to create the data basis for a tailor made management, individual grey seals will be captured on Helgoland and tagged with telemetry devices. Vocalizations of grey seals associated with mating will be recorded underwater to identify areas used for mating. In this context, a comprehensive health monitoring of grey seals will also be conducted to update knowledge on the zoonotic potential of pathogens present in grey seals for residents and tourists on Heligoland.
Additional management needs have arisen in recent years regarding the locations of grey seal births. As it repeatedly happened that single pups were born on the main island, beaches had to be closed for residents and tourists during the pupping season. At the same time, these beaches are the only places on Helgoland where dogs can run free. Thus, there is a great interest in directing the births of grey seals away from the main island, if nature conservation compatibility can be guaranteed in the process. This project will continue to investigate the extent to which the main island is already relevant during mating season.
The following questions will be addressed in the targeted project:

1) Can a seal exclusive zone be created by using an acoustic fence in the swimming area of the Helgoland dune?
2) How effective are the applied management measures on Helgoland and how can they be further optimized?
3) Which areas are used by grey seals and what are their functions? Which zoonotic pathogens do living grey seals carry on Helgoland and what danger do they pose to humans?
4) Are there possibilities to direct the locations of grey seal births?

The work packages represent the planned work required to answer the respective questions.

1) Sound exposure of grey seals in the swimming area of the Helgoland Dune.
2) Evaluation of the collected daily count data
3) Acoustic monitoring, health monitoring, and tagging of grey seals at Helgoland Dune.
4) Development of tailor made methods to keep away grey seals from main island beaches during the pupping season.

Funding: MEKUN, 229.320 EUR

Project Details:
Pressures on harbor porpoise habitat in the Schleswig-Holstein waters of the North Sea and Baltic Sea continue to increase due to anthropogenic activities. These include offshore wind turbine construction, Fehmarn Belt crossing, military and civilian blasting (munitions waste), fishing, as well as the use of PAL systems, tourism activities, chemical and pharmaceutical pollution, garbage, habitat loss, and other disturbances.
Studies of reproduction and age structure of harbor porpoises found dead in the waters of Schleswig-Holstein have shown that the animals generally die very young and that females in particular have little time to reproduce. The age structure of dead found animals provides evidence that many females die before or shortly after reaching sexual maturity. In addition, an increased number of pregnant females was found dead in the year 2021. Further data collection is needed to develop relationships that may contribute to reverse this trend. Initial studies on hearing and contaminant exposure in harbor porpoises have additionally shown that some of the animals are exposed to significant levels of persistent organic pollutants (POPs) and mercury, and hearing damage is evident in some animals. The relationships between hearing damage and exposure to contaminants as well as their significance at the population level, require further investigation. Systematic surveys of harbor porpoises are extremely important to assess trends in baseline biological data (age, sex, weight, location, date of discovery, species), as well as health status and causes of death. These data are elementary as they are reported to ASCOBANS, ICES, HELCOM, OSPAR and the IWC.
Within the framework of the project, freshly dead harbor porpoises from the Schleswig-Holstein North Sea and Baltic Sea will be examined in detail for their state of health. Histological, immunohistochemicaland microbiological examinations will be performed. The parasite fauna and its prevalence can give important information about its influence on the harbor porpoise, but also on the whole ecosystem in the Baltic Seaas intermediate hosts are important for transmission in different trophic levels. These additional investigations shall be used for the assessment parameters in the North Sea and Baltic Sea for the development of GES (Good Environmental Status) in the future and applied for OSPAR, HELCOM and the Marine Protection Framework Directive. In particular, if harbor porpoise mortalities become more frequent, immediate investigations are to be conducted to shed light on the possible causes and to uncover correlations. Based on the results, recommendations are to be developed regarding areas with increased potential for conflict and thus a further requirement for habitat investigations. Ears from well preserved animals found dead will also be collected and made available for further studies to examine the changes occuring in bycatch compared to animals found dead as well as to assess the effects of blasting, acoustic and chemical exposure. Similarly, post mortem examinations will be used to take collect samples for toxicological studies that can be analyzed as part of further projects.

Funding: UBA/BFG, 350.135 EUR

Project Details:
In the European Union, biocides are officially approved following the EU Biocide regulation No. 528/2012. So far, the following eight anticoagulants were assessed and approved for rodenticide use in the EU: Warfarin, Chlorophacinone, Coumatetralyl, Bromadiolone, Difenacoum, Brodifacoum, Difethialone and Flocoumafen. The first three active ingredients belong to the first-generation anticoagulants, whereas the last five belong tot he second-generation anticoagulants. Second-generation anticoagulant rodenticides (AR) were considered persistent, bioaccumulative and toxic (PBT- or vPvB ingredients) and thus only approved for pest control under very strict regulations, due to the lack of alternative ingredients.

Taking into account the results of previous research projects to investigate the contamination of ARs in fish and the effects of Ars on the aquatic environment, this project aims to evaluate the effectiveness of current risk mitigation measures to reduce contamination of water bodies with Ars by using a biota monitoring approach. A nationwide, strategic monitoring for otters (Eurasian otter, Lutra lutra) will be initiated to evaluate current risk mitigation and to determine relevant exposition for both aquatic and semi-aquatic top predators. In addition, the transfer of active ingredients alongside the aquatic food chain and the metabolism of Ars in fish will be investigated.

The outcome of the project with regards to environmental contamination of aquatic habitats with Ars and the effectiveness of current risk mitigation measures shall allow the funding agency to implement scientifically based decisions in the biocide registration. Also, former decisions might be revised or substantially improved.