Research

Project Details:
Das Tumormikromilieu stellt einen essentiellen Faktor sowohl beim Tumorwachstum als auch der Tumortherapie dar. Eine Komponente des Tumormikromilieus repräsentiert die Gefäßversorgung der Neoplasien, da Tumoren ohne vaskulären Anschluss ein limitiertes Größenwachstum bis zu einer kritischen Größe aufweisen, über die hinaus eine Versorgung der Zellen über Diffusion nicht mehr möglich ist. Des Weiteren weist das Tumormikromilieu infiltrierende Entzündungszellen auf, die sowohl wachstumsfördernde als auch -hemmende Effekte entfalten können. Das Tumormikromilieu stellt daher einen wesentlichen Angriffspunkt für neue therapeutische Interventionen dar. Histiozytäre Sarkome sind maligne Tumoren, die sowohl beim Hund als auch beim Menschen mit einer schlechten Prognose einhergehen und für die effektive Therapien oft nicht verfügbar sind. Daher stellt die virale Onkolyse einen faszinierenden Ansatzpunkt für die Erweiterung des Therapiespektrums dieser Neoplasie dar. Im vorliegenden Projekt soll der Einfluss einer kaninen Staupevirusinfektion auf kanine histiozytäre Sarkomzellen unter standardisierten in vitro Bedingungen als auch in einem murinen Xenotransplantationsmodell detailliert analysiert werden.

Funding: EU-Interreg, Bavarian Nordic, 270.000 EUR

Project Details:
Ticks transmit various human and animal pathogens. With regard to pathogens, the vector function of ticks for the causative agent of Lyme borreliosis (Borrelia burgdorferi sensu lato complex), human and animal granulocytic anaplasmosis and tick-borne encephalitis (TBE virus) is of particular importance. Studies on the abundance and distribution of ticks and their pathogens are therefore essential for assessing the One Health risk. Regarding tick-transmitted pathogens, this project collects data not only for Germany but also at the international level.

Results:

Rapp, J., Springer, A., Strube, C. (2024) Ixodes ricinus ticks survive flooding.  Ticks and Tick-Borne Diseases 15, 102417

 

Springer, A., Jordan, D., Höltershinken, M., Barutzki, D., Strube, C. (2024) Endemisation and management of Babesia divergens on a beef production farm. Current Research in Parasitology & Vector-Borne Diseases 6, 100188

 

Springer, A., Lindau, A., Probst, J., Fachet, K., Schäfer, I., Dobler, G., Mackenstedt, U., Strube, C. (2024) Germany-wide Citizen Science study reveals spread of Babesia canis-infected Dermacentor reticulatus ticks by dogs travelling within the country. Current Research in Parasitology & Vector-Borne Diseases 6, 100d187

 

Probst, J., Springer, A., Fingerle, V., Strube, C. (2024) Frequency of Anaplasma phagocytophilum, Borrelia spp. and coinfections in Ixodes ricinus ticks collected from dogs and cats in Germany. Parasites & Vectors 17, 87

 

Probst, J., Springer, A., Topp, A.-K., Bröker, M., Williams, H., Dautel, H., Kahl, O., Strube, C. (2023) Winter activity of questing ticks (Ixodes ricinus and Dermacentor reticulatus) in Germany − Evidence from quasi-natural tick plots, field studies and a tick submission study.  Ticks and Tick-Borne Diseases 14, 102225

Cooperation Partners:

Dr. O. Kahl, tick-radar GmbH

Prof. Dr. U. Mackenstedt, Universität Hohenheim

Dr. V. Fingerle, Nationales Referenzzentrum für Borrelien, Oberschleißheim

Prof. Dr. G. Dobler, Nationales Konsiliarlabor für FSME, München

Dr. L. Chitimia Dobler, Fraunhofer-Institut für Immunologie, Infektions- und Pandemieforschung

Project Details:
Ticks of the genus Ixodes transmit various human and animal pathogens. In this long-term project running since 2005, pathogen prevalence in ticks in the city of Hannover, Germany, is monitored to investigate potential effects of climate change. In addition to the infection status of the ticks, the tick density in the Hannover area will also be determined.

Results:

Glass, A., Springer, A., Raulf, M.-K., Fingerle, V. Strube, C. (2023) 15-year Borrelia prevalence and species distribution monitoring in Ixodes ricinus/inopinatus populations in the city of Hanover, Germany. Ticks and Tick-Borne Diseases 13, 102074

 

Glass, A., Springer, A., Strube, C. (2022) A 15-year monitoring of Rickettsiales (Anaplasma phagocytophilum and Rickettsia spp.) in questing ticks in the city of Hanover, Germany. Ticks and Tick-Borne Diseases 13, 101975

 

Hauck, D., Springer, A., Chitimia-Dobler, L., Strube, C. (2020) Two-year monitoring of tick abundance and influencing factors in an urban area (city of Hanover, Germany). Ticks and Tick-Borne Diseases 11, 101464

 

Blazejak, K., Raulf, M.-K., Janecek, E., Jordan, D., Fingerle, V., Strube, C. (2018) Shifts in Borrelia burgdorferi (s.l.) geno-species infections in Ixodes ricinus over a 10-year surveillance period in the city of Hanover (Germany) and Borrelia miyamotoi-specific Reverse Line Blot detection. Parasites & Vectors 11, 304

 

Blazejak, K., Janecek, E., Strube, C. (2017) A 10-year surveillance of Rickettsiales (Rickettsia spp. and Anaplasma phagocytophilum) in the city of Hanover, Germany, reveals Rickettsia spp. as emerging pathogens in ticks. Parasites & Vectors 10, 588

Cooperation Partners:

Dr. V. Fingerle, Nationales Referenzzentrum für Borrelien, Oberschleißheim

Funding: Fritz-Ahrberg-Stiftung, 25.000 EUR

Project Details:
Alternative ingredients such as plants or insects as substitutes for meat in (vegetarian or vegan) meat products have been increasingly researched in recent years and are already being used in some commercial products. Algae are also an interesting alternative to meat, as they are also high in protein. A distinction is made between microalgae and macroalgae. Scientific studies with microalgae species such as Chlorella spp. or Spirulina spp. as meat substitutes in meat products have shown that their addition has an influence on physicochemical parameters, especially colour. Since the studies published to date have mostly used only one microalgae species in a few concentrations with one type of meat (mostly pork), the proposed project aims to produce cooked sausages from two different microalgae species, Chlorella spp. or Spirulina spp. in different concentrations to replace the meat content, varying the type of meat replaced (pork, beef, chicken). In addition to physicochemical parameters such as pH value or colour, microbiological parameters (after inoculation, if applicable) will also be examined during storage in a protective gas atmosphere in order to evaluate the influence of meat replacement by microalgae during storage in retail outlets.

Funding: Deutsche Forschungsgemeinschaft (DFG), 83.448 EUR

Project Details:
Antibiotic resistance is a major concern in human and veterinary medicine. Particularly the rapid rise of multi-drug-resistant bacteria, spanning several antibiotic classes, challenge treatment efficacy. Simply reducing antibiotics usage is not sufficient to mitigate the crisis. To rethink the handling of antibiotics and potentially postpone or alter the evolution of antibiotic resistance, we lack understanding of the evolutionary dynamics of resistance emergence. To gain such understanding, we propose to analyze vast amounts of animal companion pathogen infection data and associated antibiotic sensitivity tests, collected by one of the leading veterinary diagnostic labs in Europe, Laboklin. In doing so we will quantify pathogen dynamics and transmission rates, cross-resistance dynamic and resistance evolution risk across many pathogens, geographic regions in Germany, and antibiotic classes. These quantifications will then be used to predict future trends in cross-resistance evolution and derive, through mathematical modelling, strategies to help alleviate the antibiotic resistance evolution crisis.

Cooperation Partners:

LABOKLIN GmbH & Co. KG

Funding: QS-Wissenschaftsfonds, 34.980 EUR

Project Details:
As part of the project, a practical feeding concept will be developed that utilizes a protein-rich by-product stream from insect production and, while taking ecological and economic aspects into account, enhances food safety by reducing zoonotic pathogens in fresh poultry products.

Funding: BB horses GmbH & Co. KG, 58.390 EUR

Project Details:
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Funding: Associations, 161.400 EUR

Funding: Gesellschaft für Kynologische Forschung (GFK), 10.000 EUR

Project Details:
Due to the frequent use of antibiotics, there are more and more diseases caused by resistant germs in both human and veterinary medicine. Unfortunately, more and more germs on our dogs' skin are showing this problem, so that skin infections that were previously easy to treat are now difficult to treat. Therefore, alternatives to antibiotics are increasingly being sought. A team led by Dr. Jessica Meißner from the Institute for Pharmacology, Toxicology and Pharmacy at the Hannover University of Veterinary Medicine Foundation is now checking whether products based on manuka honey or ozone oil can help in the fight against multi-resistant skin germs (especially Staphylococcus pseudintermedius) in dogs.

Project Details:
Aposematic warning coloration is primarily associated with deterring potential predators. However, only limited knowledge exists regarding the role of such visual signals in intraspecific communication. The aim of this study is to enhance the color intensity of a group of dorsally yellow Dendrobates tinctorius from the locality "Citronella" through dietary supplementation with carotenoids, such as β-carotene and lutein derivatives. The underlying assumption of this experiment is that coloration can be modified through carotenoid supplementation to a degree that allows supplemented individuals to be distinguished from unsupplemented ones by the naked eye, as well as through measurable shifts in reflectance spectra. Individuals exhibiting different levels of pigmentation (supplemented and supplemented = control) will be put together in a test arena for mate-choice experiments and the assessment of territorial behavior. There is reason to assume that particularly intense coloration confers selective advantages in terms of mate choice or dominance behavior, as has been suggested in previous studies on strawberry poison frogs. Such selective advantages, including increased attractiveness or greater competitive ability, are of particular interest in the context of the evolutionary mechanisms underlying warning coloration and the pronounced phenotypic variation observed among different local populations. The study will examine whether the color differences are perceptible to the frogs themselves and how individuals from the two experimental groups respond behaviorally to differently colored conspecifics. Previous studies further suggest that carotenoids may have a stimulatory effect on clutch production. To test this assumption, the reproductive performance of the separately housed experimental groups will be analyzed. The insights gained from this work may contribute to improved amphibian husbandry, provide new perspectives on intraspecific communication, and shed light on the mechanisms driving the evolution of aposematic coloration.