Equipment

Axio Imager M2 with ApoTome (Zeiss)

for shots of „confocal-like“ fluoreszenz pictures with highly reduced diffused light
on an anti-vibration-basis. 

Fluoreszenz-light:   HXP120 white light source
transmitted light:   Halogen-lamp HAL 100

Via the black/white camera the pictures can be transferred directly into the Software „ZEN2 blue“ to regard and work on them.
Additional color camera for histology.

Available objektives:
Plan Apochromat 10x / 0,3
Plan Apochromat 63x / 1,4 Oil  
Plan Apochromat 20x / 0,8
Plan Apochromat 100x/ 1,4 Oil
Plan Apochromat 40x / 0,95 with correcting ring

Available filtersets:
38-> excitation BP 470/40 ; beamsplitter FT 495 ; emission BP 525/50
43-> excitation BP 550/25 ; beamsplitter FT 570 ; emission BP 605/70
49-> excitation G365          ; beamsplitter FT 395 ; emission BP 445/50
50-> excitation BP 640/30 ; beamsplitter FT 660 ; emission BP 690/50

Chemidoc MP (Biorad)

with RYB-Light, Fluorescence and Chemiluminescence
Possibility of transmitted light with white light convertion screen

For detection of DNA (syBr-green,…), proteins (Coomassie, silver staining or stainfree) and fluorescence dyes

Digitization of the images and evaluation

FACS Attune NxT with Autosampler

FACS analysis for single-cell-suspensions of fixed and non-fixed S2-samples.
Compatible with classical FACS-tubes or Eppis as well as with automatic samplic of different plates (96 well etc.).
Attune works based on hydrodynamic and additional acustic focussing.
Possible throughput rate:  1 ml/min

Excitation  Laser violett (405nm) -> Detection 440/50 ; 512/25 ; 603/48 ; 710/50 
Excitation Laser blue (488nm)     -> Detection  530/30 ; 590/40 ; 695/40 
Excitation  Laser yellow (561nm) -> Detection  585/16 ; 620/15 ; 695/40 ; 780/60 
Excitation  Laser red (637nm)      -> Detection  670/14 ; 720/30 , 780/60  

Special pump mechanistics guarantee the input of specified quantities of sample volumes. Samples not used can be recycled and fed back.

FACS Fortessa X20

FACS analysis for single-cell-suspensions of non-infectious S2-samples.
Compatible with BD FACS-tubes as well as with S2-samples with automatic samplic system HTS  for plates (96 well).
Separation of signals highly efficient by mirror System

Excitation  Laser violett (405nm) -> Detection 780/60; 710/50; 670/30; 610/20; 525/50; 450/50
Excitation  Laser red (640nm)      -> Detection 780/60; 730/45; 670/30
Excitation Laser blue (488nm)     -> Detection 780/60; 710/50; 610/20; 575/25; 530/30

Microscope with Climate Chamber for Life-Cell-Imaging (Nikon)

Fluorescence mikroscope TE 300 (NIKON) with climate chamber, invert and motorized for life cell imaging.

Climate chamber CO₂ and temperature adjustable.
Holder for different plates and slides.
Via the black/white camera the pictures can be transferred directly into the Software „NIS“ to regard and work on them.   

Available objektives:

Plan Fluor            10x / 0,3
S-Plan Fluor         20x / 0,45 EL WD
S-Plan Fluor         40x / 0,6 EL WD
S-Plan Fluor         60x / 1,4 
additional x1,5 optical magnification available for all objectives

Available filtersets:

EX: 377/50   DM:409     BA: 447/60 BP  (DAPI)
EX: 472/30   DM:495     BA: 520/35 BP  (FITC, EGFP)
EX: 562/40   DM:593     BA: 624/40 BP  (Alexa568, mCherry, TxRed)
EX: 628/40   DM:660     BA: 692/40 BP  (Cy5)
EX: 545/25   DM:565     BA: 605/70 BP  (Cy3)

INFINITE F200 PRO OR SPARC (TECAN)

Plate reader with filters for absorption, immunofluorescence, polarisation and luminescence (Luziferase assay).
Adjustable and heatable chamber, one with gas and climate chamber (CO₂ and N₂)  for hypoxia conditions.
Injection of reagents before and during measurement possible.

All chambers can be temperated (->+42°C) and shaking

ZZ first floor:
with injector -> possibility to add liquids during measurements
Absorption (optic can 230 – 1000nm), filter for: 405(10), 450(10), 595(10), 620(10)
Fluorescence: (optic 230 – 850nm), filter for: 485(50)/ 535(50)
incl bottomreading and F. Polarisation
Luminescence module for luciferase-assay and Multicolor luminescence, Filter 1+2 in green, Magenta, Blue1, Green1

ZZ 2^nd floor:
with gas (CO2 + N2), humidity chamber and lid lifter for long term measurement
Absorption Monochromator 200-1000 nm (each nm possible)
Fluorescence: (optic 230 – 850nm), Filter for Ex: 380(20), 485(20)/ 550(10) (Combination free to choose), Filter for Em: 535(25), 620(20), 670(40), 460(20), incl bottomreading (No fluorescence polarization, no luminescence)

FI S3:    
Absorption (optic can 230 – 1000nm), filter for: 450Ex, 405Ex, 620Ex, 595Ex
Fluorescence (optic 230 – 850nm), filter for 485(20)/535(25), 550(10)/620(20), 400(20)/505(20)
incl bottomreading and F. Polarisation
Luminescence module for luciferase-assay and Multicolor luminescence
Injector

FI S3e: 
Absorption Monochromator 200-1000 nm (each nm possible)
Fluorescence: (optic 230 – 850nm), we have filter for excitation 560(20), 485(20), 495(10), 430(20), 360(35) and for emission 620(10), 535(25), 540(25), 485(20), 465(35), 520(10) incl bottomreading (No fluorescence polarization)
Luminescence (no multicolor)
Injector

Multiscan

only for Absorptions of 200-100 nm

O2 Control in vitro Glove Box (Coy)

For Tissue Culture.

Perform incubation, culture maintenance & analysis in the same environment.

Qiacube - Automatic Extraction of DNA & RNA

Up to 12 columns (Qiagen systems).

Ideal conditions for comparable handling of different samples allowing reproducable results in less time. Possible for isolation of high quality DNA and RNA from different samples like blood, tissue or gels.

Qiacube HT roboter

Extraction of DNA, RNA and more on a 96-well-format from different types of sampes (tissue, blood) for Qiagen-kits "Qiacube HT".

Parallel processing of up to 96 samples (units of 8).
Vacuum system with a column insert for high throughput and standardised conditions in less time.

Light cycler 96 (Roche)

96-well format

Aria MX (Agilent)

96-well format

Ultracentrifuge Optima XPN (Beckman)

rotors: 
70Ti                 Max 70.000UPM/504.000xg           8 x 35,5 ml
SW40Ti            Max 40.000UPM/285.000xg           6 x 14 ml
SW60Ti            Max 60.000UPM/485.000xg           6 x 4 ml
SW32Ti            Max 32.000UPM/175.000xg           6 x 38,5 ml

Avanti-J-26S XP (Beckman)

big centrifuges also for larger amounts of liquids

Rotors:          
JA25.50          Max.25.000 UPM/75.600xg      8 x  50 ml
JLA16.250      Max.16.000 UPM/38.400xg      6 x 250 ml
JLA10.500      Max.10.000 UPM/18.600xg      6 x 500 ml