Research

Funding: Niedersächsisches Ministerium für Ernährung, Landwirtschaft und Verbraucherschutz, 255.930 EUR

Project Details:
The mouflon or mouflon Ovis gmelini musimon only occurs in a few remaining populations in Lower Saxony. The mouflon should have a good chance of survival at least in some low mountain regions due to its predator avoidance strategy with short steep escapes up rocky slopes, but has become extinct in the lowland regions due to the presence of the wolf.
There is much discussion about the few animals in Lower Saxony and Germany in general: The mouflon is not originally native here (not autochthonous) and causes high levels of damage to forestry locally. On the other hand, it is classified as endangered by the IUCN in its native Corsica and Sardinia and is a symbolic species for hunters. Therefore, in this study we want to take a neutral view and use photo traps and direct observations to determine facts about abundance, behavior and enemy avoidance strategies as well as damage effects. In addition, surveys and interviews will be used to obtain the opinions of the various stakeholders (hunters, foresters, farmers, other nature lovers, etc.).
All of this data will be used to objectify the discussion about mouflon, large predators and human interests and to model the survival probabilities of Lower Saxony's mouflon populations in the presence of wolves and lynx.

Funding: IDT Biologika und von der Coalition for Epidemic Preparedness Innovations (CEPI), 484.000 EUR

Project Details:
We will study the immunogenicity and efficacy of our vaccine candidate, MVA-MERS-S, when used in different single vaccination and vaccination-booster setting in the lethal k18-hDPP4-mouse model after MERS-CoV-challenge infection. Different vaccination schedules also include different time points of challenge infection. We will characterize the activation of MERS-CoV-specific antibodies and T cells. The protective efficacy will be analyzed by morbidity, mortality and viral load in the lung and the brain a target organs. Moreover, a histopathological examination of the upper and lower respiratory tract of the animals upon challenged with MERS-CoV will show any evidence of ADE and ERD, respectively. Another study will evaluate the impact of passively transfered sera from MVA-MERS-S vaccinated mice for the outcome of protection after challenge infection.

Funding: Niedersächsisches Ministerium für Ernährung, Landwirtschaft und Verbraucherschutz, 104.438 EUR

Project Details:
From 12.04. to 15.04.2023 the "Black Grouse Symposium 2023, Will the black grouse survive in the Lüneburg Heath?" took place at Camp Reinsehlen in Schneverdingen. The event was organized by the Alfred Toepfer Academy for Nature Conservation (NNA), the ITAW, the Lower Saxony Hunting Association (LJN), the Lower Saxony Ministries of Agriculture (ML) and Environment (MU) and the Nature Conservation Park Association (VNP). At the symposium, the alarming state of the black grouse population in Lower Saxony, known and suspected causes of this state and possible solutions were discussed. The participants, in particular the local stakeholders from the areas of occurrence, clearly advocated a species aid program for the black grouse in the Lüneburg Heath, which should provide a framework for eliminating the problems discussed in the best possible way and achieving a healthy, stable population in the medium to long term.

At a follow-up event initiated by the Lower Saxony Ministry of Food, Agriculture and Consumer Protection in June 2023, the project to develop a black grouse action plan was launched. The project is being implemented by the ITAW and the NNA. The contents of the action plan will be coordinated with the involved stakeholders from landowners, authorities and other interest groups as part of a "round table".

Cooperation Partners:

Projektpartner: Alfred Toepfer Akademie für Naturschutz (NNA)

Funding: BUND Landesverband Nidersachesen e.V., 37.018 EUR

Project Details:
Underwater noise from ship traffic, the construction of facilities in the water and on the coast or the explosion of ammunition in the sea is identified as a threat by various national and international expert assessments and has negative effects on marine animals in the Wadden Sea - especially on marine mammals, including porpoises. Many projects on the effects of noise pollution on marine mammals underwater are already being carried out at the ITAW. The ITAW is particularly concerned with the effects of underwater noise on harbor porpoises, with a particular focus on ship traffic, offshore constructions, and unexploded ordnance contaminated sites (e.g. BfN-UWE2, BMBF-COASTALFUTURE, EU project SATURN). In this joint project with BUND, ITAW will provide support with recommendations for measures to reduce the effects of underwater noise on porpoises, development of voluntary rules of conduct, participation in panel discussions and materials for exhibitions. Different target groups should be made aware of the need to reduce underwater noise through different offers (exhibitions, lectures in schools, raising awareness among recreational boat owners). At the same time, recommendations for concrete measures to reduce underwater noise pollution are being developed.

Results:

(BUND) Leaflet containing information on the sources of underwater noise pollution caused by recreational boats and on the measures that recreational boaters and sailors can take to help protect porpoises.

https://www.bund-niedersachsen.de/service/publikationen/detail/publication/ruhe-fuer-die-schweinswale/

Funding: Niedersächsisches Ministerium für Wissenschaft und Kultur, 178.000 EUR

Project Details:
Measure 1 consists of work package A, which is part of e-learning service. The use of the blended learning format in veterinary teaching is to be promoted. A further focus will be placed on strengthening the (e-)didactic skills of lecturers and the digital skills of students, for which training courses will be created for these target groups.

Measure 1 also includes work package B, which is located in the Clinical Skills Lab. The establishment of a surgical logbook is intended to strengthen students' surgical skills. To support the implementation of the surgical logbook, a training concept for teaching surgical skills is being developed.

Funding: Bundesministerium für Bildung und Forschung, 625.000 EUR

Project Details:
As long as animal experiments cannot be completely replaced, the question will arise on which animals research should take place. In this project, an interdisciplinary working group wants to investigate the ELSA-relevant question, according to which criteria the selection of laboratory animal models takes place or should take place. The aim of the enterprise is to determine the manifold reasons for the choice of certain laboratory animals; the process is interdisciplinary in the full sense of the word and put the different rationalities into a well-founded correlation. The question condenses several open and important issues concerning the treatment of animals. The answer is to be examined in each of the disciplines involved, between them and finally in their association, whether it is possible to establish a coherent catalog of criteria that also creates clarity for the practice of animal experimentation, both for everyday research and at the interface to legislation and enforcement. This is important against the background of rapidly changing social value judgements about animals, which also lead to permanent tensions here. In this project we want to investigate the ELSA-relevant question of the criteria for the selection of laboratory animal models. We follow an interdisciplinary approach, which is represented by the following cooperation partners: Its members are: Prof. Dr. Peter Kunzmann (ethics), Prof. Dr. Bernhard Hiebl (laboratory animal science), Prof. Dr. Maren von Köckritz-Blickwede (infection research) (all TiHo- Hannover) and the legal scientist Prof. Dr. Dr. Tade Spranger (University of Bonn).

Cooperation Partners:

Tade Spranger, Rechts- und Staatswissenschaftliche Fakultät, Bonn

Funding: DFG, 498.400 EUR

Project Details:
The equine recurrent uveitis (ERU) is the main reason for blindness in horses, and 10% of the european horses are affected. Significantly, ERU is an important available model for human autoimmune uveitis as the clinical and immune-pathological characteristics are similar. As a cause for ERU, various hypotheses are described in the literature. On the one hand, infections with Leptospira interrogans are discussed as well as other bacterial, parasitic or viral infections. Independent of the initiating cause, there are numerous indications that ERU is an immune-mediated disease. In the body after the control of the primary infection, an overreaction of the immune system occurs in response to activated immune cells. In addition to lymphocytes, these immune cells can also be neutrophil granulocytes. Among various antimicrobial functions, neutrophils have been shown to release neutrophil extracellular traps (NETs). NETs are extracellular DNA fibers with associated proteases and antimicrobial peptides which are released by activated cells. NETs mediate entrap of invading pathogens, but when massively released may contribute to detrimental autoimmune reactions of the host.
The research project presented here is intended to provide essential insights for the involvement of NETs in the pathogenesis of ERU. In the first phase of our study, our findings indicate an involvement of NETs in the pathogenesis of ERU with detrimental effects. In short, NETs correlate to disease severity, seem to damage the blood retinal cells, and induce autoantibodies against NET proteins. We hypothesize, that those autoantibodies might shield the NETs from degradation by DNases, resulting in their persistence and potentially in new disease episodes. Thus, in the current project we aim to characterize if and how the phase of disease (non-active quiescent phase versus acute inflammatory phase) influences cellular involvement and extracellular DNA release. Based on our previous data, we further hypothesize that different cell types besides neutrophils play a role in extracellular DNA release during the progression of ERU, which enables crosstalk between various immune cells. Therefore, we will study the release of ETs by other immune cells besides neutrophils and its interplay with adaptive immune cells. Finally, since the use of gentamicin in the treatment of ERU patients is well established, the aim is to determine the influence of gentamicin in varying concentrations and along with distinct stimuli on equine immune cells, focusing on ET release.
 

Funding: Niedersächsisches Ministerium für Wissenschaft und Kultur (MWK), 324.000 EUR

Project Details:
The aim of this subproject is the further development and optimization of a 3D lung epithelial cell culture model
for the investigation of viral and bacterial infections and the effect of immunomodulatory
immunomodulatory substances on the course of infection and cell biochemical processes. An increased
complexity of the model system using physiological or pathophysiological oxygen
conditions and microfluidic systems enables the in vitro system to be approximated to the in vivo situation and
to the in vivo situation and will help to reduce the number of test animals in the long term. The
complexity of the infection model is reduced by physiologically relevant oxygen conditions (defined
tissue-specific hypoxic conditions) and by 3D co-cultivation of human or animal pulmonary
or animal pulmonary epithelial cells and neutrophil granulocytes. In addition
the use of primary cells and induced pluripotent stem cells should provide long-term optimization.

Funding: Bundesanstalt für Landwirtschaft und Ernährung (BLE), 134.128 EUR

Project Details:
The overall goal of the project is that farms that have high antibiotic use (ABU) can learn from farms that have comparably low ABU and the identification and optimization of parameters to improve animal health. The specific objectives of the project are: to improve biosecurity and overall hygiene of broiler chicken farms, to improve animal health of broiler chickens, to minimize the use of antibiotics, to transfer knowledge between broiler chicken farms with low and high ABU, and to optimize risk areas based on knowledge transfer.
There should be an intensive exchange of experiences between farmers who have different levels of ABU within the project. Thus, farms that have a high need for antibiotic use at the beginning of the project will learn which parameters they can and should optimize to improve animal health and minimize antibiotic use. Farm management factors that have a potential impact on animal health and consequent necessary ABU will be evaluated and collected during the practical phases of the project. These factors are divided into respective work packages (WP), including biosecurity of the farm and hygiene management (worked on by FLI & UROS), cleaning and disinfection (UROS), animal health and stable or herd management (TiHo and LMU), and knowledge transfer (UROS).
The project thus involves farms that have already been able to minimize their antibiotic use through innovative measures, optimized management and improved husbandry conditions. In addition, farms that are interested in improving animal health and minimizing antibiotic use and want to learn from other farms are invited to participate.

Cooperation Partners:

Uni ROS; Prof. Helen Louton

LMU München; PD Dr. Elke Rauch

FLI Insel Riems; PD Dr. Nicolai Denzin

Funding: Umweltbundesamt, 306.051 EUR

Project Details:
Plastic pollution is now known worldwide and is an omnipresent problem for every ecosystem. In 2021, the annual plastic production was 390.7 million tonnes. A distinction is made between macroplastics (> 5 mm), microplastics (MP; < 5 mm) and nanoplastics (in the nanometre range).
These plastic particles enter the marine environment via various routes, e.g. via wastewater, atmospheric transport, ocean currents, landfills and fishing activities. Once in the environment, macro litter is broken down into smaller particles because the polymer structure is weakened by photodegradation (UV), hydrolysis, mechanical abrasion, biodegradation (microorganisms) or biofouling (colonisation of plastics by microbes).
The polar regions were long considered to be largely unaffected by humans. Even though it is not a heavily populated region, MP has been detected in this ecosystem in a variety of compartments such as the water column, snow, sea ice and deep-sea sediments. The increase in temperature as a result of climate change and the associated melting of ice is causing various problems - in addition to the rise in sea level, the increasing decrease in sea ice is leading to the release of microplastics that were previously bound by the ice deposits. This is now "available" and enters the sensitive ecosystem - the consequences cannot yet be estimated. The presence of microplastics in the Arctic means that species in the polar region are now increasingly confronted with an anthropogenic problem - the presence of marine litter or microplastics and its consequences. This also includes Arctic peoples, whose diet also includes the meat and fatty tissue of marine mammals.
The aim of the project is to obtain valid data on microplastics and associated pollutants in marine mammals from Arctic waters and thus improve the state of knowledge in this sensitive habitat. The acquired knowledge is the basis for future trend analyses that are necessary to enable effective MP monitoring in Arctic waters. The following species characteristic of the Arctic will be studied: harbour porpoise (Phocoena phocoena), ringed seal (Pusa hispida), grey seal (Halichoerus grypus), harbour seal (Phoca vitulina), hooded seal (Cystophora cristata), bearded seal (Erignathus barbatus) and polar bear (Ursus maritimus). Another important aspect is the establishment of a method for visualising MPs in tissue. For this purpose, pieces of tissue from the gastrointestinal tract are prepared and examined both histologically and using a confocal microscope. Various staining techniques are then used to identify potential microplastics in the tissue. This is an important step towards better understanding the transport routes of microplastics and verifying entry paths.
The samples come from various co-operation partners such as the University of Iceland and the Norwegian Polar Institute. The pollutant analysis is being carried out by the University of Siena.