Research

Project Details:
Ein unspezifisches chronisches Krankheitsgeschehen wurde mit einer Toxikoinfektion mit Clostridium botulinum (sog. viszeraler/chronischer Botulismus) in Verbindung gebracht. Unter anderem wurde von typischen Lahmheitssymptomen berichtet. In einer Fall-Kontroll-Studie konnte ein Zusammenhang zwischen dem chronischen Krankheitsgeschehen und Clostridium botulinum nicht nachgewiesen werden. Chronisch kranke Tiere aus Kontroll- und Fallbetrieben waren aber vermehrt lahm, wobei die Lahmheit überwiegend durch Erkrankung an den Klauen zustande kam. In vorliegendem Projekt wird untersucht, ob sich lahme Kühe aus Kontroll- und Fallbetrieben hinsichtlich der Diagnosen an den Klauen unterscheiden. In einer Risikofaktorenanalyse wurden weiterhin verschiedene Faktoren aus Haltung, Fütterung und Management auf mögliche Beziehungen zur Anzahl der Klauendiagnosen pro Tier untersucht.

Cooperation Partners:

Vereinigte Informationssysteme Tierhaltung w.V.(vit)

Funding: 5.000 EUR

Project Details:
Die Tierärztliche Hochschule Hannover hat von der Landwirtschaftlich-Gärtnerischen Fakultät der Humboldt-Universität Berlin eine Miniaturschweinepopulation (Mini-LEWE) übernommen, die sich vom Göttinger Miniaturschwein unterscheidet. Die Sauen, Eber und deren Nachkommen sollen regelmäßig klinisch und labordiagnostisch hinsichtlich verschiedener Gesundheitsparameter untersucht werden.
Die Anpaarungen werden entsprechend der Abstammung und genetischen Diversität optmimiert.
Die Mini-Lewe Population ist eine sehr wichtige Ressource für biomedizinische Versuche und eine erhaltungswürdie Population.
Die genetische Charakterisierung dieser Population erfolgt über populationsgenetische Kennzahlen zur Verwandtschaft, Inzuchtzunahme, effektive Populationsgröße, Inzuchtgrad und erwartete Inzuchtzunahme. Tiefergehende Analysen beruhen auf genomweiten Markersystemen, Beadchip-Genotypisierungen und Komplettgenomanalysen. Diese Daten geben Einblick in die genomische Architektur, genomische Verwandtschaft, genomische Diversität, Selektionssignaturen und ROH-Inseln.
Für spezifische Versuche werden Haplotypen von Genclustern und Gengruppen charakterisiert, um Tiere für Versuche auswählen zu können.

Results:

Reimer et al. 2018. Analysis of porcine body size variation using re-sequencing data of miniature and large pigs. BMC Genomics. 2018 Sep 19;19(1):687. doi: 10.1186/s12864-018-5009-y.

 

Schachler et al. Schätzung der genetischen Diversität der Mini-Lewe Zuchtpopulation und Einfluss von Inzucht auf Wurfgrößenmerkmale

Züchtungskunde, 91, (3) S. 227-245, 2019, ISSN 0044-5401

https://elib.tiho-hannover.de/receive/etd_mods_00000392?q=Richel

Cooperation Partners:

Prof. Dr. Rainer Blasczyk, MHH

Prof. Dr. Sabine Hammer, Vetmed Uni Wien

Funding: Bundesinstitut für Risikobewertung, Berlin

Project Details:
The project VetCAb (Veterinary Consumption of Antibiotics) is a research project that is carried out to describe the use of antibiotics in farm animals in Germany. In 2007 and 2008 the project was conducted as a feasibility study and subsequently in 2011 a pilot study in a cross-sectional approach. The pilot study not only documented the consumption of antibiotics based on of individual farm information, but also directly related it to the livestock, so that a risk assessment can be realized. This defined a status quo for Germany, which made it possible to determine measures to reduce the use of antibiotics in farm animals.

Building on this, the VetCAb-Sentinel project is continued from 2013 as a longitudinal study with ongoing participant recruitment and data collection. The aim of the study is to evaluate how often the animals are treated with antibiotics during a defined period of time based on the treatment frequency (TF), i.e. # treated animals x # treatment days / # animals per farm. In addition, the study will, among other things, investigate whether regions or farm sizes differ with regard to the use of antibiotics and which classes of active substances are used in German livestock farming. VetCAb-Sentinel thus forms the basis of a scientific risk assessment, which, according to the DART (Deutsche Antibiotika Resistenzstrategie), makes a significant contribution to the reduction of antibiotic resistance.

https://ibei.tiho-hannover.de/vetcab

Results:

https://ibei.tiho-hannover.de/vetcab/

https://ibei.tiho-hannover.de/vetcab/pages/41

Project Details:
Unsere Hypothese ist, dass die endoskopische Besamung im Vergleich zur üblichen manuell-transvaginalen Besamung in den Gebärmutterkörper eine graduell verstärkte reaktive Entzündungsreaktion verursacht. Hierzu soll die reaktive Entzündungsreaktion des Endometriums (mit und ohne Samenübertragung) durch versuchsbegleitende Probengewinnung (Endometriumabstriche für bakteriologische und zytologische Untersuchungen, Uterusbiopsie zur pathohistologischen Untersuchung des Endometriums) verglichen werden.

Results:

doi: 10.1016/j.jevs.2020.103023. PMID: 32534786

Cooperation Partners:

Niedersächsisches Landgestüt Celle

Institut für Veterinär-Pathologie, Universität Leipzig

Funding: Alexander von Humboldt Stiftung Mohammed Bin Zayed Species Conservation

Project Details:
The genus Eleutherodactylus, with over 194 known species and a distribution centered in the Caribbean West Indian islands, is the most speciose lineage of the Eleutherodactylidae family. These frogs represent over 80% of the Caribbean amphibian diversity with most of the species being single-island endemics. With the aid of multiple collaborators, this long term research project seeks to: 1) uncover cryptic diversity and define the species limits in the genus Eleutherodactylus by integrating genomic and phenotypic information; 2) elucidate the phylogenetic relationships among the species and related taxa; 3) compare the phenotypes, geographic distributions and evolutionary history across species to identify the factors responsible for the evolutionary diversification of amphibians in an insular scenario; and 4) generalize the results towards the long term conservation of these species.

Cooperation Partners:

Dr. Roberto Alonso Bosch, University of Havana, Cuba

MSc. Manuel Iturriaga Monsibay, Institute of Ecology and Systematics, Cuba

Prof. Dr. Miguel Vences, Technische Universität Braunschweig, Germany

Dr. Carles Vilà, Estación Biológica de Doñana, CSIC, Spain

Dr. Alejandro Gonzalez Voyer, Instituto de Ecología, UNAM, Mexico

Álvaro Dugo Cota, Estación Biológica de Doñana, CSIC, Spain

Funding: Private Personen und Kliniken Zuchtverbände, 30.000 EUR

Project Details:
Chromosomenmutationen können die Ursache von Fruchtbarkeitsstörungen, Intersexualität oder Missbildungen bei unseren Haussäugetieren sein. In diesen Fällen kann eine Chromosomendarstellung, zum Beispiel aus den Blutlymphozyten oder aus einer Gewebekultur, zur Klärung der Ursache beitragen.
Neben den klassischen Methoden der Zytogenetik zur Detektion von Chromosomenmutationen bei Einzeltieren werden zudem Gene mittels einer Fluoreszenz in situ Hybridisierung physikalisch am Genom der verschiedenen Spezies kartiert.

Results:

Iannuzzi A, Braun M, Genualdo V, Perucatti A, Reinartz S, Proios I, Heppelmann M, Rehage J, Hülskötter K, Beineke A, Metzger J, Distl O., Clinical, cytogenetic and molecular genetic characterization of a tandem fusion translocation in a male Holstein cattle with congenital hypospadias and a ventricular septal defect. PLoS One. 2020 Jan 10;15(1):e0227117. doi: 10.1371/journal.pone.0227117. eCollection 2020.

Funding: Nieders. Ministerium für Wissenschaft und Kultur aus Mitteln des Niedersächsischen Vorab der Volkswagenstiftung, 12.900 EUR

Project Details:
The funding was used for the EU project application to prepare a pre-proposal for a joint transnational project which we were able to submit on time on May 18, 2020. Our project, with the acronym ASSESSOR, addresses Theme 1 - Measuring Environmental Behavior (Workpackages 2-4) and Theme 2 - Evaluation and Risk Assessment (Workpackages 5-6) of CECs, pathogens, and antimicrobial-resistant bacteria from aquatic ecosystems, including population-level modeling of impacts on aquatic top predators and humans. To achieve this goal, we propose a work plan consisting of seven work packages, each addressing coordination (work package 1), corresponding analyses (work packages 2-5), risk assessment (work package 6), and communication (work package 7).

Results:

A corresponding project application was submitted on time, but unfortunately could not be funded.

Funding: Bundesamt für Naturschutz, 46.404 EUR

Project Details:
The aim of the project was to check whether there are indications that harbor porpoises were harmed and affected by the blasting of ground mines from the 2nd World War, which took place in the area of the nature reserve "Fehmarnbelt". For this purpose, harbor porpoises and their body parts, which were collected in Schleswig-Holstein, Mecklenburg-Vorpommern and Denmark and were still available, were examined for health damage in relation to effects of blasting. The aim was to check not only for immediate lethal damage, but also for sublethal changes. These can result in porpoises being less able to find food and orient themselves. This in turn increases the risk of becoming ill, as well as being exposed to collision trauma or bycatch. Here, the exclusion procedure was used to evaluate whether the findings that were collected were caused by other causes. With the agreement of MELUND, data collected during the annual harbor porpoise mortality monitoring were included in an assessment of this particular issue.

Results:

Siebert, U., Stürznickel, J., Schaffeld., T., Oheim, R., Rolvien, T., Prenger-Berninghoff, E., Wohlsein, P., Lakemeyer, J., Rohner, S., Schick, L.A., Gross, S., Nachtsheim, D., Ewers, C., Becher, P., Amling, M., Morell, M. 2022. Blast injury on harbour porpoises (Phocoena phocoena) from the Baltic Sea after explosions of deposits of World War II ammunition. Environment International 159, 107014. https://doi.org/10.1016/j.envint.2021.107014

https://doi.org/10.1016/j.envint.2021.107014

Funding: Ministerium für Energiewende, Landwirtschaft, Umwelt, Natur und Digitalisierung, 45.525 EUR

Project Details:
For several years, hunters in Schleswig-Holstein have observed an increase in diseased and deceased hares, depending on the region, as well as a decrease in hunting distances. The results of the spotlight taxations also show that despite relatively constant spring populations, the hares counted during the autumn taxations have been decreasing for several years. For this reason, 190 hunting hares from selected hunting grounds, as well as 79 fall hares from all over Schleswig-Holstein, have already been examined in detail for infectious diseases as a potential cause for the decline in the hare population in 2016 - 2019. Histological, virological, parasitological and microbiological samples were taken from each animal in addition to collecting physiological data. The occurrence of tularemia (Francisella tularensis), as well as Rabbit Haemorrhagic Disease Virus Type 2 (RHDV-2) and European Brown Hare Syndrome Virus (EBHSV) was checked in cooperation with specialized institutes. In this way, we have already been able to gain an impression of the incidence of infection over a number of years and have identified territory-specific differences, which we are now attempting to clarify further.
The results of the investigations so far show above all changes in the digestive organs, an increased exposure to parasites and indicate a high infestation with EBHSV. In the past year of investigation, RHDV2 was detected for the first time in rabbits from Schleswig-Holstein. Although high mortality rates after infection with this virus have been shown in rabbits in other countries, RHDV2 has not been detected in any rabbits in this study. Whether rabbits play a role as a source of infection for hares in Schleswig-Holstein will also be investigated in more detail.
As in previous years, the results will be evaluated together with the Landesjagdverband S.-H. and the Wildtierkataster S.-H., whereby further investigation strategies or precautionary measures will be developed. This project supports the further development of preventive health protection and food safety measures for wild animals in Schleswig-Holstein.

Results:

For several years the hunters of Schleswig- Holstein observed an increase of sick an dead european brown hares (Lepus europaeus). Therefore the Institute for Terrestrial and Aquatic Wildlife Research (ITAW) of the University of Veterinary Medicine Hannover in cooperation with the Wildtierkataster Schleswig- Holstein (WTK) of the University of Kiel, as well as the Landesjagdverband Schleswig- Holstein (LJV- SH) started in 2016 to examine european brown hares for infectious diseases. The study was funded for four years by the Ministerium für Energiewende, Landwirtschaft, Umwelt, Natur und Digitalisierung des Landes Schleswig- Holstein (MELUND).

With the support of the hunters of Schleswig- Holstein it was possible to examine in total 356 european brown hares during the study in the years 2016, 2017, 2019 and 2020. We dissected the hares and histological, virological, parasitological and microbiological samples were taken from each animal. The occurrence of tularemia (Francisella tularensis), as well as Rabbit Haemorrhagic Disease Virus Type 2 (RHDV-2) and European Brown Hare Syndrome Virus (EBHSV) was examined in cooperation with specialized institutes.

In comparison, in the year 2020 less pathological findings were diagnosed than in 2016 and 2017, but more than in 2019. The gastrointestinal tract of the hares was affected the most and and an increased exposure with parasites was confirmed. We also found a lot of hares with subclinical hepatitis and follicular hyperplasia of the spleen, which could be due to an infection with the european brown hare virus (EBHSV), as antibody titers confirmed that almost all hares that we examined were in contact with the virus. EBHSV is highly contagious and causes a high mortality which could lead to a massive decline of the population.

Like in the former years we diagnosed steatitis and nephritis in multiple hares, the origin of these pathological findings is not confirmed yet.

In 2020 stomach content and livers of 10 hares were examined at the Institute or Animal Nutrition of the University of Veterinary Medicine Hannover for the quanitity of vitamine E and unsaturated fatty acids. The number of examined animals was really low, but the results indicate that the steatitis could be caused by a vitamine E deficiency while having an excess of unsaturated fatty acids. Futher studies need to be done to confirm this.

To find the reason for the decline of the european brown hare population multidisciplinary research is needed as there are multiple factors that cause the decrease. By studying the health status of the european brown hares for multiple years we have been able to gain an impression of the occurance of infectious diseases in the population of Schleswig- Holstein.

file:///C:/aaNeueWebseiten/DozIS%20Projekte/2021/Ergebnisse%202021/Feldhasen_Studie_Infekionsstatus_2020_Klink.pdf

Funding: Ministerium für Energiewende, Landwirtschaft, Umwelt, Natur und Digitalisierung, 53.210 EUR

Project Details:
In this research project, ITAW will investigate infectious diseases, the state of health and the causes of death due to illness and changes in harbour seals in Schleswig-Holstein. The research project will investigate pathological changes in connection with infectious agents, with a special focus on zoonotic diseases. The occurrence of virological, microbiological and parasitological pathogens will be investigated intensively. The project is expected to provide valuable data for the seal populations that continue to grow in the North Sea. The scientific findings are to be passed on to the seal hunters so that they are trained in the detection of infectious diseases and in the handling of marine mammals. Development and review of hygiene measures, infectious diseases and documentation materials play an important role in this. A regionally representative number of seals is selected from those passed on by seal hunters. These are intensively researched and assessed for the presence of diseases.
These examinations include a complete autopsy of the animals, further examinations such as histology, bacteriology, virology, serology and parasitology, age determination as well as taking and securing samples for later analyses or research projects. Furthermore, a statistical analysis on the size of the necessary sample for statements on the state of health is to be carried out. A scientific exchange with the seal hunters will take place during the entire research project period.

Results:

Studies on the health status of harbour seals in Schleswig-Holstein in 2020

Report to the Ministry for Energy Transition, Agriculture, Environment, Nature and Digitalisation of the State of Schleswig-Holstein and the State Agency for Coastal Protection, National Park and Marine Conservation Schleswig-Holstein (only German)

https://www.tiho-hannover.de/fileadmin/57_79_terr_aqua_Wildtierforschung/79_Buesum/downloads/Berichte/Seehunde_Gesundheitsmonitoring_2020.pdf