Antibiotic use and bacterial resistance
In various projects, the working group is investigating the influence of antibiotics on the development of bacterial resistance of commensal and pathogenic bacteria. Especially, bacteria from farm animals (beef, pigs, chickens) and pets (including dogs) living in close contact with humans play an important role in the studies. In addition, alternatives to antibiotics are investigated, as well as possibilities to reduce antibiotic susceptibility of various bacteria.
Implant material, nanoparticles
The working group is involved in two DFG-funded research projects in which the suitability of different alloys as implant material is investigated. In addition, experiments to optimize the surface finish of such implants using various in-vitro models are conducted. Tests on cell and tissue compatibility (biocompatibility) are carried out in various cell culture systems and isolated organs. These examinations are supplemented by biofilm examinations on the implant surface. In another DFG-funded project, the biocompatibility and the accumulation of drug-loaded magnetic nanoparticles caused by magnetic fields are investigated. After accumulation in the corresponding tissue, these nanoparticles should enable a targeted release of active substance and thus prevent or reduce infections at the implant site.
Immunopharmacology
Allergic and autoimmune diseases are caused by dysregulation of the immune system, which leads to misguided reactions to harmless environmental antigens or endogenous proteins. The focus of our working group is the skin, as this organ is particularly frequently affected, e.g. in atopic dermatitis in dogs or psoriasis in humans. In the studies, the members of the working group are concentrating on the role of the antigen-presenting cells (dendritic cells) and keratinocytes, because these cell types are of crucial importance for the development and maintenance of the diseases. A main focus is on the question of whether modulators of the histamine-4 receptor can influence the allergic inflammatory process. For this purpose, various inflammation models of the skin are characterized in more detail.
Transdermal penetration, permeation and absorption
Comparative in-vitro and ex-vivo studies on transdermal penetration, permeation and absorption are carried out using suitable model substances. In addition to studies on non-perfused skin of various species in diffusion cells (e.g. horse, dog, pig), experiments on perfused skin are also carried out using the isolated perfused bovine udder, which allow predictive statements with regard to pharmacological and toxicological issues.
Isolated perfused sheep limbs as alternatives to animal experiments
Taking into account the 3R concept, the model of the isolated perfused ovine limb is under establishment in the working group and examined with regard to its suitability as a substitute and supplementary method for animal experiments. Various viability parameters and the possibility of releasing inflammatory cytokines are examined.
