In vitro methods exchange

To facilitate the search for contacts for in vitro methods at the TiHo, working groups that have established the listed methods in their laboratories are listed here.

 

Institute for Food Quality and Food Safety, Research Group Seeger

  • Differentiation of human induced pluripotent stem cells (hiPSCs) into different neuronal target cells (e.g. motor neurons, sensory neurons, brain spheres)
  • Generation of intestinal organoids from hiPSCs or intestinal stem cells isolated from the intestine of different species
  • Cell culture of cell lines from different species

Performance of various tests in cell culture

  • activity assay of botulinum toxins in motor neurons differentiated from human induced pluripotent stem cells (hiPSCs) (Western blot, luminescence assay)
  • cytotoxicity assays and cell proliferation assays (MTT, WST, LDH, ...)
  • assays for the detection of enzyme activities (CYP1A1, CYP2B1, ...)
  • characterization of barrier properties in intestinal epithelial cells via measurement of transepithelial resistance (TEER) and via fluorescence markers (Lucifer-Yellow) in transwell inserts
  • Western blot (cleavage of SNARE proteins, HIF1-alpha expression,...)
  • Immunofluorescence staining (neuron-specific markers, markers for specific cells of the intestinal epithelium)
  • RT-qPCR (expression of differentiation markers, inflammatory markers,...)
  • Flow cytometry (stem cell markers, differentiation markers,...)
  • ELISA (cytokine release,...)

Contact:
Prof. Bettina Seeger, Ph.D. (bettina.seeger(at)tiho-hannover.de; +49 511-856-7602)

Institute for Pharmacology, Toxicology and Pharmacy, Research Group Löscher

  • In vitro models of the blood-brain barrier (BBB) of different species (human, mouse, rat, pig), both immortalized cell culture and primary cell culture
  • Cultivation of the in vitro BBB models in the transwell system and investigation of barrier properties by measuring transendothelial resistance (TEER) and using radioactively or fluorescently labeled paracellular tracers
  • Investigation of protein (Western blot) and gene expression (qPCR) and cellular protein localization (fluorescence microscopy, live cell imaging, organelle preparation) after drug exposure
  • Investigation of mechanisms of drug resistance and pharmacological intervention strategies (functional assays: activity measurement of the drug transporter P-glycoprotein, transport assays across the BBB)
    Isolation of brain capillaries from tissue

Contact:
Prof. Dr. Wolfgang Löscher (wolfgang.loescher(at)tiho-hannover.de; Tel. +49 511-953-8729)

Institute for Pharmacology, Toxicology and Pharmacy, Research Group Meißner

  • Franzzell diffusion experiments
  • Isolated perfused bovine udder
  • Measurement of cytokine release (cell culture and tissue sections)
  • Precision udder slices
  • Precision lung slices
  • Proliferation assays
  • Viability assays
  • Cell culture of murine dendritic cells, macrophages and osteoblasts to study biocompatibility of implants, nanoparticles and other test substances
  • Cell migration assays

Contact:
Dr. Jessica Meißner (jessica.meissner(at)tiho-hannover.de; +49 511-953-8723)

Institute for Physiology and Cell Biology, Division of Cell Biology

  • In vitro assay for developmental neurotoxicity on a human neuronal progenitor cell line (NT-2)
  • In vitro assay for developmental neurotoxicity on locust embryos
  • Resazurin-based cell viability assays with fluorescence microplate reader (Tecan Infinite 200)

Contact:
PD Dr. Miachel Stern (michael.stern(at)tiho-hannover.de; +49 511-856-7767)

Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, Research Group Hiebl

  • Hemocompatibility testing of substances
  • Cytotoxicity testing
  • Microsurgical procedures on laboratory animals

Contact:
Prof. Dr. Bernhard Hiebl (bernhard.hiebl(at)tiho-hannover.de; +49 511-856-8958