In vitro methods exchange

To facilitate the search for contacts for in vitro methods at the TiHo, working groups that have established the listed methods in their laboratories are listed here.

 

Institute for Food Quality and Food Safety, Research Group Seeger

  • Differentiation of human induced pluripotent stem cells (hiPSCs) into different neuronal target cells (e.g. motor neurons, sensory neurons, brain spheres)
  • Generation of intestinal organoids from hiPSCs or intestinal stem cells isolated from the intestine of different species
  • Cell culture of cell lines from different species

Performance of various tests in cell culture

  • activity assay of botulinum toxins in motor neurons differentiated from human induced pluripotent stem cells (hiPSCs) (Western blot, luminescence assay)
  • cytotoxicity assays and cell proliferation assays (MTT, WST, LDH, ...)
  • assays for the detection of enzyme activities (CYP1A1, CYP2B1, ...)
  • characterization of barrier properties in intestinal epithelial cells via measurement of transepithelial resistance (TEER) and via fluorescence markers (Lucifer-Yellow) in transwell inserts
  • Western blot (cleavage of SNARE proteins, HIF1-alpha expression,...)
  • Immunofluorescence staining (neuron-specific markers, markers for specific cells of the intestinal epithelium)
  • RT-qPCR (expression of differentiation markers, inflammatory markers,...)
  • Flow cytometry (stem cell markers, differentiation markers,...)
  • ELISA (cytokine release,...)

Contact:
Prof. Bettina Seeger, Ph.D. (bettina.seeger(at)tiho-hannover.de; +49 511-856-7602)

Institute for Pharmacology, Toxicology and Pharmacy, Research Group Meißner

  • Franzzell diffusion experiments
  • Ex vivo pyoderma skin model
  • Isolated perfused bovine udder
  • Isolated perfused equine and ovine limb
  • Precision-cut udder slices (PCBUS, cattle)
  • Precision-cut lung slices (PCLS, mouse)
  • Uterus explants (cattle)
  • Primary cell isolation from cadavers of various species and organs
    • Murine dendritic cells, macrophages, and osteoblasts (e.g. for biocompatibility testing of implants, nanoparticles, and other test substances)
    • Dermal fibroblasts and keratinocytes (dog, bovine, equine, mouse)
    • Bovine mammary epithelial cells (from udder tissue or milk)
    • Bovine uterine epithelial cells
    • Ocular cells (endothelial, stromal, and epithelial cells) from cattle and dog
  • Proliferation assays
  • Viability assays
  • Biocompatibility testing: Measurement of cytokine release (cell culture and tissue sections),Western blot analyses (e.g. p38), Alizarin red staining, Alkaline phosphatase (ALP) activity assays, Live/dead assays, ROS measurement
  • Cell migration assays (murine dendritic cells)
  • Co-culture of bacteria with cells or tissues to study infection pathways and interactions

Contact: PD Dr. Jessica Meißner (jessica.meissner(at)tiho-hannover.de; +49 511-953-8723)

Institute for Physiology and Cell Biology, Division of Cell Biology

  • In vitro assay for developmental neurotoxicity on a human neuronal progenitor cell line (NT-2)
  • In vitro assay for developmental neurotoxicity on locust embryos
  • Resazurin-based cell viability assays with fluorescence microplate reader (Tecan Infinite 200)

Contact:
PD Dr. Miachel Stern (michael.stern(at)tiho-hannover.de; +49 511-856-7767)

Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, Research Group Hiebl

  • Hemocompatibility testing of substances
  • Cytotoxicity testing
  • Microsurgical procedures on laboratory animals

Contact:
Prof. Dr. Bernhard Hiebl (bernhard.hiebl(at)tiho-hannover.de; +49 511-856-8958