Adamek, Mikolaj Dr. MSc.


 

Wissenschaftlicher Mitarbeiter

Dr. ing. agrar., Fischereiwissenschaften

 

Tel: 0511 953-8579

Fax: 0511 953-8587

 

e-mail schreiben

Projekte und Aufgaben

  • Modeling of the interaction between type I IFN responses and survival of CyHV-3 and SVCV infections in carp.
  • Influence of cholesterol on CyHV-3 and SVCV infections.
  • CyHV-3 experimental infections and in vitro culture based prediction of survival.
  • Development of in vitro skin (epithelium) model – characterization of cell contacts and mucins in carp and turbot.
  • Molecular evaluation of skin and tank bacterial microflora in carp culture.
  • Antimicrobial activity of antimicrobial peptides in trout and carp semen.
  • Development of siRNA treatment for CyHV-3.
  • Molecular diagnostics (AngHV-1, CEV, ISKNV).
  • Diagnostic assay development.

Forschungsinteressen

  • Fish innate immune responses to viral infections in particularly type I interferon system
  • Mucosa immunity and skin barrier modeling - studies on junction proteins, mucins, antimicrobial peptides
  • Immunogenetic background of disease resistance
  • Host - pathogen interactions
  • Development of diagnostic tests for the detection of novel fish pathogens

Wissenschaftlicher Werdegang

Education

 

 

PhD level 2005-2013: University of Agriculture in Krakow, Faculty of Animal Breeding and Biology  Department of Ichthyobiology and Fisheries transferred in 2011 to Inland Fisheries Institute in Olsztyn due to formal changes in Department of Ichthyobiology and Fisheries;

Doctor of Agricultural Sciences in the field of Fisheries awarded with the distinction by the Inland Fisheries Institute on 30.08.2013
Title of Doctoral thesis: The role of type I interferon on immune responses of carp infected with Cyprinid herpesvirus 3.

                                              

MRes level 2006-2007: Keele University, School of Life Sciences, MRes in European Scientific Research Training.

 

Title of Master of Research awarded on 14.11.2007. Thesis title: Interferon type I system of common carp (Cyprinus carpio) and its influence on Cyprinid herpesvirus 3 (CyHV-3) infection in vitro.   

 

MEng level, 2000-2005: University of Agriculture in Krakow, Faculty of Animal Breeding and Biology Specialization: Fisheries and Water Environment Protection.

 

Title of Master of Engineering awarded on 08.07.2005 Thesis title: Gene polymorphism of MHC class IIB and resistance on bacterial infection of Cyprinus carpio with Aeromonas hyprophila.

 

 

Professional Experience

 

15.08.2011 –  :Research Associate, Fish Disease Research Unit,

University of Veterinary Medicine Hannover, Germany

 

Tasks:

  • Modeling of the interaction between type I IFN responses and survival of CyHV-3 and SVCV infections in carp.
  • Influence of cholesterol on CyHV-3 and SVCV infections.
  • CyHV-3 experimental infections and in vitro culture based prediction of survival.
  • Development of in vitro skin (epithelium) model – characterization of cell contacts and mucins in carp and turbot.
  • Molecular evaluation of skin and tank bacterial microflora in carp culture.
  • Antimicrobial activity of antimicrobial peptides in trout and carp semen.
  • Development of siRNA treatment for CyHV-3.
  • Molecular diagnostics (AngHV-1, CEV, ISKNV).
  • Diagnostic assay development.
  • Teaching. Master and Bachelor student supervision.

 

 

15.08.2009 – 14.08.2011: Experienced Researcher Marie Curie Initial Training Network NEMO, Fish Disease Research Unit,

University of Veterinary Medicine Hannover, Germany

 

Tasks:

  • Development of CyHV-3 experimental infections model.
  • Evaluation type I IFN and apoptotic responses during CyHV-3 and SVCV infections.
  • Influence of beta-glucan feeding on antimicrobial peptides and microflora in carp.

 

15.07.2005 – 14.08.2009 : PhD student, Department of Ichtiobiology and Fisheries

Faculty of Animal Breeding and Biology, University of Agriculture in Krakow

                                                         

Tasks:

  • Teaching.

 

Experimental work

15.07.2005 – 14.08.2009, PhD student, Polish Academy of Sciences,

Institute of Ichthyobiology & Aquaculture in Golysz

 

Tasks:

  • Evaluation of immunogenetic background of the resistance of common carp to CyHV-3 infections.
  • Influence of type I IFN responses and gene polymorphism on the resistance of carp to CyHV-3 infections.
  • CyHV-3 diagnostics.

            

Other Experience

 

Workshops

 

5-7.12.2005                              DNA Analysis by Automatic Sequencer, Museum and Institute of Zoology, Polish Academy of Sciences, Warsaw, Poland

 

11-15.04.2010                           Fish Immunology Workshop, Wageningen University, Nederland

 

16-18.06.2010                           Molecular Epidemiology, University of Veterinary Medicine in Hanover, Germany

 

15-17.09.2010                           An essential guide to 3-dimensional protein structure and its determination, Keele University UK

 

25-27.10.2010                           Microarrays – results analysis, MBS Service for Molecular Biology, Warsaw Poland

 

7-8.05.2011                              Grantcraft & Grantmanship – Proposal Writing Skills GrantCraft WRG Europe Ltd. Krakow Poland

 

9-11.05.2011                             Common carp as a model organism for biological studies, Institute of Ichthyobiology and Aquaculture in Golysz Poland

 

14-15.11.2011                           Frontiers in protein science, Technical University of Denmark, Copenhagen Denmark

Publikationen

Chyb J. Mikolajczyk T. Sokolowska-Mikolajczyk M. Socha M. Szczerbik P. Biedroń D. Dębowska M. Kasiuk M. Madej J. Podczerwińska E. Adamek M. Epler P. (2008) Effects of non-steroidal gonadal factors on LH secretion in female common carp during the reproductive cycle. Czech Journal of Animal Science, 53, 398-403.

 

Rakus K.Ł. Wiegertjes G.F. Adamek M. Bekh V. Stet R.J.M. Irnazarow I. (2008) Application of PCR-RF-SSCP to study major histocompatibility class II B polymorphism in common carp (Cyprinus carpio L.). Fish & Shellfish Immunology, 24, 734-744.

 

Rakus K.Ł. Wiegertjes G.F., Adamek M. Siwicki A.K. Lepa A. Irnazarow I. (2009) Resistance of common carp (Cyprinus carpio L.) to Cyprinid herpesvirus-3 is influenced by major histocompatibility (MH) class II B gene polymorphism. Fish & Shellfish Immunology, 26, 737-43.

 

van der Marel M.* Adamek M.* Gonzalez S.F. Frost P. Rombout J.H.W.M. Wiegertjes GF, Steinhagen D. (2012) Molecular cloning and expression of two B-defensin and two mucin genes in common carp (Cyprinus carpio L.) and their up-regulation after B-glucan feeding. Fish & Shellfish Immunology, 32, 494-501. *Joint first authors. 

 

Rakus K.Ł. Irnazarow I. Adamek M. Palmeira L. Kawana Y. Hirono I. Kondo H. Matras M. Steinhagen D. Flasz B. Brogden G. Vanderplasschen A. Aoki T. (2012) Gene expression analysis of common carp (Cyprinus carpio L.) lines during Cyprinid herpesvirus 3 infection yields insights into differential immune responses. Developmental & Comparative Immunology, 37, 65-76.

 

Adamek M. Rakus K.Ł. Chyb J. Brogden G. Huebner A. Irnazarow I. Steinhagen D. (2012) Interferon type I responses to virus infections in carp cells: in vitro studies on Cyprinid herpesvirus 3 and Rhabdovirus carpio infections. Fish & Shellfish Immunology, 33, 482-93.

 

Brogden G. von Köckritz-Blickwede M. Adamek M. Reuner F. Jung-Schroers V. Naim H.Y. Steinhagen D. (2012) β-glucan protects neutrophil extracellular traps against degradation by Aeromonas hydrophila in carp (Cyprinus carpio). Fish & Shellfish Immunology, 33, 1060-4.

 

Syakuri H. Adamek M. Brogden G. Rakus K.Ł. Matras M. Irnazarow I. Steinhagen D. (2013) Intestinal barrier of carp (Cyprinus carpio L.) during a Cyprinid Herpesvirus 3-infection: Molecular identification and regulation of the mRNA expression of claudin encoding genes. Fish & Shellfish Immunology, 34, 305-314.

 

Adamek M. Syakuri H. Harris S. Rakus K.Ł. Brogden G. Matras M. Irnazarow I. Steinhagen D. (2013) Cyprinid herpesvirus 3 infection disrupts the skin barrier of common carp (Cyprinus carpio L.). Veterinary Microbiology, 162, 456-470.

 

Baums C.G. Hermeyer K. Ohlemeyer S. Adamek M. Czerny C.P. Hörstgen-Schwark G. Valentin-Weigand P. Baumgärtner W. Steinhagen D. (2013) Establishment of a Model of Streptococcus iniae Meningoencephalitis in Nile Tilapia (Oreochromis niloticus). Journal of Comparative Pathology,149(1):94-102.

 

Syakuri H. Jung-Schroers V. Adamek M. Brogden G. Steinhagen D. (2014) Beta-glucan feeding differentiated the regulation of mRNA expression of claudin genes and prevented an intestinal inflammatory response post Aeromonas hydrophila intubation in common carp, Cyprinus carpio L. Journal of Fish Diseases, 37, 149–156.

 

Brogden G. Propsting M. Adamek M.  Naim H.Y. Steinhagen D. (2014) Isolation and analysis of membrane lipids and lipid rafts in common carp (Cyprinus carpio L.). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 169, 9–15.

 

Adamek M. Steinhagen D. Irnazarow I. Hikima J. Jung T.S. Aoki T. (2014) Biology and host response to Cyprinid herpesvirus 3 infection in common carp. Developmental & Comparative Immunology, 43, 151–159.

 

Brogden G. Krimmling T. Adamek M.  Naim H.Y. Steinhagen D. von Köckritz-Blickwede M. (2014) The effect of β-glucan on formation and functionality of neutrophil extracellular traps in carp (Cyprinus carpio L.). Developmental & Comparative Immunology, 44, 280-285.

 

Huang Y. Adamek M. Walker C. Runge M. Steinhagen D. (2014) In vitro cytotoxicity and multiplex PCR detection of virulence factors of Yersinia ruckeri isolated from rainbow trout in North West Germany. Berliner und Münchener Tierärztliche Wochenschrift, 127(5-6):233-42.

 

Pionnier N. Adamek M. Miest J.J. Harris S.J. Matras M. Rakus K.Ł. Irnazarow I. Hoole D. (2014) CRP and complement as acute phase reactants in common carp Cyprinus carpio during CyHV-3 infection. Diseases of Aquatic Organisms, 109, 187-199.

 

Adamek M. Rakus K.Ł. Brogden G. Matras M. Chyb J. Hirono I. Kondo H. Aoki T. Irnazarow I. Steinhagen D. (2014) Interaction between type I interferon and Cyprinid herpesvirus 3 in two different genetic lines of common carp (Cyprinus carpio L.). Diseases of Aquatic Organisms, 111, 107–118

 

Dietrich M.A. Adamek M. Bilińska B. Hejmej A. Steinhagen D. Ciereszko A. (2014) Characterization, expression and antibacterial properties of apolipoproteins A from carp (Cyprinus carpio L.) seminal plasma. Fish & Shellfish Immunology, 41, 389-401.

 

Adamek M. Rauch G. Brogden G. Steinhagen D. (2014) Small interfering RNA treatment can inhibit Cyprinid herpesvirus 3 associated cell death in vitro. Polish Journal of Veterinary Sciences, 17 (4), 733-735.

 

Miest J.J. Adamek M. Pionnier N. Harris S. Matras M. Rakus K.Ł. Irnazarow I. Steinhagen D. Hoole D. (2015) Differential effects of Alloherpesvirus CyHV-3 and Rhabdovirus SVCV on apoptosis in fish cells. Veterinary Microbiology, 176 (2015) 19–31.

 

Jung-Schroers V.* Adamek M.* Teitge F. Hellmann J. Bergmann S.M. Kleingeld D.W. Way K. Stone D. Runge M. Keller B. Hesami S. Waltzek T. Steinhagen D. (2015) Another potential carp killer? – Carp Edema Virus disease in Germany. BMC Veterinary Research 2015, 11:114 *Joint first authors.

 

Brogden G.* Adamek M.* Propsting M. Reiner U. Naim H.Y. Steinhagen D. (2015) Cholesterol-rich lipid rafts play important role in Cyprinid herpesvirus 3 replication cycle. Veterinary Microbiology, 179, 204–212. *Joint first authors.

 

Dietrich M.A. Nynca J. Adamek M. Steinhagen D. Karol H. Ciereszko A. (2015) Expression of apolipoprotein A-I and A-II in rainbow trout reproductive tract and their possible role in antibacterial defence. Fish & Shellfish Immunology, 45, 750-756.

 

Jung-Schroers V. Adamek M. Jung A. Harris S. Dóza Ö.S. Baumer A. Steinhagen D. (2016) Feeding of β-1,3/1,6-glucan increases the diversity of the intestinal microflora of carp (Cyprinus carpio). Aquaculture Nutrition, doi: 10.1111/anu.12320.

 

Fabian M. Baumer A. Adamek M. Steinhagen D. (2016) Transmission of Cyprinid herpesvirus 3 by wild fish species - results from infection experiments. Journal of Fish Diseases, doi: 10.1111/jfd.12399.

 

Miest J.J. Arndt C. Adamek M. Steinhagen D. Reusch T. (2016) Dietary β -glucan (MacroGard®) enhances survival of first feeding turbot (Scophthalmus maximus) larvae by altering immunity, metabolism and microbiota. Fish and Shellfish Immunology, 48, 94-104

 

 

Fabian M. Baumer A. Adamek M. Steinhagen D. (2016) Transmission of Cyprinid herpesvirus 3 by wild fish species - results from infection experiments. Journal of Fish Diseases, 39, 625–628.

 

 

Jung-Schroers V. Adamek M. Jung A. Harris S. Dóza Ö.S. Baumer A. Steinhagen D. (2016) Feeding of β-1,3/1,6-glucan increases the diversity of the intestinal microflora of carp (Cyprinus carpio). Aquaculture Nutrition, 22, 1026–1039

 

 

Adamek M. Jung-Schroers V. Hellmann J. Teitge F. Bergmann S.M. Runge M. Kleingeld D.W. Way K Stone D.M. Steinhagen D. (2016) Concentration of carp edema virus (CEV) DNA in koi tissues affected by koi sleepy disease (KSD). Diseases of Aquatic Organisms, 119, 245–251

 

 

Jung-Schroers V. Adamek M. Wohlsein P. Wolter J. Wedekind H. Steinhagen D. (2016) First outbreak of an infection with infectious spleen and kidney necrosis virus (ISKNV) in ornamental fish in Germany. Diseases of Aquatic Organisms, 119, 239–244.

 

 

Steinhagen D. Jung-Schroers V. Adamek M. (2016) Impact of cyprinid herpesvirus 3 (Koi herpesvirus) on wild and cultured fish. CAB Reviews, 2016, 11, 041, pp 1-10

 

 

Dietrich M.A. Słowińska M. Karol H. Adamek M. Steinhagen D. Hejmej A. Bilińska B. Ciereszko A. (2017) Serine protease inhibitor Kazal-type 2 is expressed in the male reproductive tract of carp with a possible role in antimicrobial protection. Fish & Shellfish Immunology 60:150-163.

 

 

Adamek M. Oschilewski A. Wohlsein P. Jung-Schroers V. Teitge F. Dawson A. Gela D. Piackova V. Kocour M. Adamek J. Bergmann S.M. Steinhagen D. (2017) Experimental infections of different carp strains with the carp edema virus (CEV) give insights into the infection biology of the virus and indicate possible solutions to problems caused by koi sleepy disease (KSD) in carp aquaculture. Veterinary Research 48, 12

 

 

Boley A. Korshun G. Boley S. Jung-Schroers V. Adamek M. Steinhagen D. Richter S. (2017) A new reactor for denitrification and micro-particle removal in recirculated aquaculture systems. Water Science and Technology, 75(5), 1204-1210

 

 

Kullmann B. Adamek M. Steinhagen D. Thiel R. (2017) Anthropogenic spreading of anguillid herpesvirus 1 (AngHV 1) by stocking of infected farmed European eels, Anguilla anguilla (L.), in the Schlei fjord in northern Germany Journal of Fish Diseases, DOI: 10.1111/jfd.12637

 

 

Nynca J. Dietrich M.A. Adamek M. Steinhagen D. Bilińska B. Hejmej A. Ciereszko A. (2017) Purification, characterization and expression of transferrin from rainbow trout seminal plasma. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 2017 208–209:38-46

 

 

Nynca J. Adamek M. Ciereszko A. (2017) Identification of differentially expressed proteins in testicular semen of sex-reversed female (XX) and normal male (XY) rainbow trout. J. Anim. Sci. 0. doi:10.2527/jas.2017.1517

 

 

Adamek M. Matras M. Jung-Schroers V. Teitge F. Heling M. Bergmann S.M. Reichert M. Way K. Stone D.M. Steinhagen D. (2017) Comparison of PCR methods for the detection of genetic variants of carp edema virus. Diseases of Aquatic Organisms. https://doi.org/10.3354/dao03152