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Neurogastroenterology

 

Team

  • Prof. Dr. Gemma Mazzuoli-Weber, Leiterin
  • Kristin Elfers, PhD, Postdoc
  • Dr. Sandra Bader, Postdoc
  • Susanne Hoppe, Technical assistant
  • Yvonne Ambrecht, Animal Caretaker
  • Anna Katharina Filzmayer, Doctoral Student
  • Laura Menne, Doctoral Student
  • Julia Stoffregen, Student assistant

Research

 

For many years it is known that the enteric nervous system (ENS) can function independently from the central nervous system. It is able to regulate all gastrointestinal functions in isolation. However, precise data on the neuronal circuits involved in the control of those functions are missing.

Up to 16 organ-bath experiments can be carried out in parallel.
Ultrafast-Neuroimaging-Setup used for live-cell imaging in the enteric nervous system.

My research group was able to describe and characterize mechanosensitive enteric neurons. These neurons possess soma and multiple neurites, which have mechanosensitive properties. Moreover we showed that neurites can have both afferent and efferent function. Mechanosensitive enteric neurons (shortly MEN) are multifunctional: they are at the same time able to sense mechanical stimuli and to drive muscle responses, which are the basis of enteric motility patterns. We showed that MEN have different phenotypes, are tension and or compression sensitive and they all receive fast synaptic input. This implies that they are part of a network which activity is highly subject to neuronal and non-neuronal modulations.

 

The concept of multifunctional MEN is valid across all gastrointestinal regions (stomach, small and large intestine) and species (rodents, human) tested.

 

Equipment

  • Ultrafast-Neuroimaging-Set up for Live-Cell-Imaging with voltage and calcium sensitive dyes
  • Macroscop for wide field Ca2+-Imaging  
  • Set up for afferents recordings
  • 16-Channels-Organ bath
  • 4-Channels-Voltage Clamp Ussing Chamber-System
  • Immunoflurescence Microscope

Research of the gastrointestinal functions and their regulation:

Secretion:

Voltage-Clamp-Ussing

This technique measure the property of permeability of epithelial tissues. Thus, transport and barrier functions of the living tissue can be detected and quantified.

 

Through the addition of substances that either enhance or inhibit secretion we can identify cellular mechanisms, neuronal circuits, neurotransmitters and -receptors or ion channels.

 

 

Motility:

In vitro motility with organ bath

 

With force transducers the contractility of isolated forestomach, stomach and intestine preparations is examined in vitro. This model is excellently suited for pharmacological studies, whereby 16 separate organ baths can be used at the same time. This method allows to study the effect of different substances on muscle activity under basal and stimulated conditions.

 

Neurotransmission:

Neuroimaging with calcium and voltage sensitive dyes

Changes in membrane potential and intracellular calcium levels can be registered using voltage and calcium sensitive dyes.
To detect these fast events, cameras with an enormously high (> 1 kHz) frame rate are required. The advantage of using optical methods in neurophysiology lies in the fact that entire populations of neurons can be studied simultaneously and network interactions can be elucidated.

 

 

Immunhistochemistry/Immunofluorescence

Enteric neurons express various neurotransmitters that yield the neurochemical code of a cell. This coding characterizes the function of the neurons. Our goal is to fully decode this encoding in the ENS along with the associated cells. With the coding already known, it has been possible to detect and specify plasticity and disease-associated changes in the enteric nervous system.

 

Colon (Mouse)

Rumen (Bovine)

Kontakt
Tel.:+49 511 856-7543
Fax.:+49 511 856-827543
Cover March 2019
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