Horse Genome Project

The first assembly of the horse genome sequence was made available by the Broad Institute. The University of Veterinary Medicine Hannover and the Helmholtz Centre for Infection Research are developing a physical map of the horse genome. Our ultimate goal is to create genome-wide BAC-contigs and a high-resolution physical map that will improve the accuracy of whole genome sequence assemblies. As a first step towards the envisioned physical map we generated 314,972 BAC end sequences from the CHORI-241 library. All BAC end sequences are publicly available from the EMBL nucleotide database and a large proportion is also deposited in the TraceArchive. We ran the end sequences through a bioinformatics pipeline that allowed the comparative mapping of horse BAC clones with respect to the human genome (BLASTN against the human genome NCBI build 36.2, threshold E ≤ 10-5). Using the comparative mapping information and the human gene annotation, the putative gene content of the equine BAC clones was inferred. Additionally the equine chromosome assignment of the BAC clones was predicted based on the currently available human-horse comparative maps.

Contacts:
Prof. Dr. Ottmar Distl
Jörn Wrede (only technical aspects)
Reference (Leeb et al. 2006, Genomics 87:772-776)
Search for the BLASTN result of a specific equine BAC clone:
Clone name:

Search for equine BES BLASTN hits within a specific human region:
Human chromosome:
Interval: -

Search for equine BAC clones that contain a specific gene

Gene symbol:


(This search will list horse BAC clones that correspond to an interval in the human genome, where a gene is annotated. The listed clones will not necessarily contain the entire query gene as clones that contain only parts of the query gene will be listed as well.)

We provide the complete comparative mapping data as compressed Excel-spreadsheets that can be downloaded using the links shown below.

This research is supported by a grant from the Volkswagen Stiftung.